DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Haeyoun | ko |
dc.contributor.author | Lee, Yunkyung | ko |
dc.contributor.author | Park, Soon A. | ko |
dc.contributor.author | Lee, Ji Hyeon | ko |
dc.contributor.author | Park, Junseong | ko |
dc.contributor.author | Park, Jang Hyun | ko |
dc.contributor.author | Lee, Heung Kyu | ko |
dc.contributor.author | Kim, Tai-Gyu | ko |
dc.contributor.author | Jeun, Sin-Soo | ko |
dc.contributor.author | Ahn, Stephen | ko |
dc.date.accessioned | 2022-11-14T05:00:09Z | - |
dc.date.available | 2022-11-14T05:00:09Z | - |
dc.date.created | 2022-11-14 | - |
dc.date.created | 2022-11-14 | - |
dc.date.created | 2022-11-14 | - |
dc.date.issued | 2022-12 | - |
dc.identifier.citation | ONCOIMMUNOLOGY, v.11, no.1 | - |
dc.identifier.issn | 2162-4011 | - |
dc.identifier.uri | http://hdl.handle.net/10203/299554 | - |
dc.description.abstract | Adoptive transfer of gamma delta T cells is a novel immunotherapeutic approach to glioblastoma. Few recent studies have shown the efficacy of gamma delta T cells against glioblastoma, but no previous studies have identified the ligand-receptor interactions between gamma delta T cells and glioblastoma cells. Here, we identify those ligand-receptor interactions and provide a basis for using gamma delta T cells to treat glioblastoma. V gamma 9V delta 2 T cells were generated from peripheral blood mononuclear cells of healthy donors using artificial antigen presenting cells. MICA, ULBP, PVR and Nectin-2 expression in 10 patient-derived glioblastoma (PDG) cells were analyzed. The in vitro cytokine secretion from the gamma delta T cells and their cytotoxicity toward the PDG cells were also analyzed. The in vivo anti-tumor effects were evaluated using a U87 orthotopic xenograft glioblastoma model. Expression of ligands and cytotoxicity of the gamma delta T cells varied among the PDG cells. IFN-gamma and Granzyme B secretion levels were significantly higher when gamma delta Tcells were co-cultured with high-susceptible PDG cells than when they were co-cultured with low-susceptible PDG cells. Cytotoxicity correlated significantly with the expression levels of DNAM-1 ligands of the PDG cells. Blocking DNAM-1 resulted in a decrease in gamma delta T cell-mediated cytotoxicity and cytokine secretion. Intratumoral injection of gamma delta T cells showed anti-tumor effects in an orthotopic mouse model. Allogenic gamma delta T cells showed potent anti-tumor effects on glioblastoma in a DNAM-1 axis dependent manner. Our findings will facilitate the development of clinical strategies using gamma delta T cells for glioblastoma treatment. | - |
dc.language | English | - |
dc.publisher | TAYLOR & FRANCIS INC | - |
dc.title | Human allogenic gamma delta T cells kill patient-derived glioblastoma cells expressing high levels of DNAM-1 ligands | - |
dc.type | Article | - |
dc.identifier.wosid | 000876712000001 | - |
dc.identifier.scopusid | 2-s2.0-85141356461 | - |
dc.type.rims | ART | - |
dc.citation.volume | 11 | - |
dc.citation.issue | 1 | - |
dc.citation.publicationname | ONCOIMMUNOLOGY | - |
dc.identifier.doi | 10.1080/2162402X.2022.2138152 | - |
dc.contributor.localauthor | Lee, Heung Kyu | - |
dc.contributor.nonIdAuthor | Choi, Haeyoun | - |
dc.contributor.nonIdAuthor | Lee, Yunkyung | - |
dc.contributor.nonIdAuthor | Park, Soon A. | - |
dc.contributor.nonIdAuthor | Lee, Ji Hyeon | - |
dc.contributor.nonIdAuthor | Park, Junseong | - |
dc.contributor.nonIdAuthor | Kim, Tai-Gyu | - |
dc.contributor.nonIdAuthor | Jeun, Sin-Soo | - |
dc.contributor.nonIdAuthor | Ahn, Stephen | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Gamma delta T cells | - |
dc.subject.keywordAuthor | glioblastoma | - |
dc.subject.keywordAuthor | adoptive cell transfer | - |
dc.subject.keywordAuthor | PVR (CD-155) | - |
dc.subject.keywordAuthor | Nectin-2 | - |
dc.subject.keywordAuthor | DNAM-1 receptor (CD266) | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | RADIOTHERAPY | - |
dc.subject.keywordPlus | DISPERSAL | - |
dc.subject.keywordPlus | EFFICACY | - |
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