DC Field | Value | Language |
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dc.contributor.author | Koh, June -Young | ko |
dc.contributor.author | Rha, Min-Seok | ko |
dc.contributor.author | Choi, Seong Jin | ko |
dc.contributor.author | Lee, Ha Seok | ko |
dc.contributor.author | Han, Ji Won | ko |
dc.contributor.author | Nam, Heejin | ko |
dc.contributor.author | Kim, Dong-Uk | ko |
dc.contributor.author | Lee, Jae Geun | ko |
dc.contributor.author | Kim, Myoung Soo | ko |
dc.contributor.author | Park, Jun Yong | ko |
dc.contributor.author | Park, Su-Hyung | ko |
dc.contributor.author | Joo, Dong Jin | ko |
dc.contributor.author | Shin, Eui-Cheol | ko |
dc.date.accessioned | 2022-11-07T02:01:15Z | - |
dc.date.available | 2022-11-07T02:01:15Z | - |
dc.date.created | 2022-11-07 | - |
dc.date.created | 2022-11-07 | - |
dc.date.created | 2022-11-07 | - |
dc.date.issued | 2022-10 | - |
dc.identifier.citation | JOURNAL OF HEPATOLOGY, v.77, no.4, pp.1059 - 1070 | - |
dc.identifier.issn | 0168-8278 | - |
dc.identifier.uri | http://hdl.handle.net/10203/299322 | - |
dc.description.abstract | Background & Aims: The liver provides a unique niche of lym-phocytes enriched with a large proportion of innate-like T cells. However, the heterogeneity and functional characteristics of the hepatic T-cell population remain to be fully elucidated.Methods: We obtained liver sinusoidal mononuclear cells from the liver perfusate of healthy donors and recipients with HBV-associated chronic liver disease (CLD) during liver trans-plantation. We performed a CITE-seq analysis of liver sinusoidal CD45+ cells in combination with T cell receptor (TCR)-seq and flow cytometry to examine the phenotypes and functions of liver sinusoidal CD8+ T cells.Results: We identified a distinct CD56hiCD161-CD8+ T-cell population characterized by natural killer (NK)-related gene expression and a uniquely restricted TCR repertoire. The fre-quency of these cells among the liver sinusoidal CD8+ T-cell population was significantly increased in patients with HBV-associated CLD. Although CD56hiCD161-CD8+ T cells exhibit weak responsiveness to TCR stimulation, CD56hiCD161-CD8+ T cells highly expressed various NK receptors, including CD94, killer immunoglobulin-like receptors, and NKG2C, and exerted NKG2C-mediated NK-like effector functions even in the absence of TCR stimulation. In addition, CD56hiCD161-CD8+ T cells highly respond to innate cytokines, such as IL-12/18 and IL-15, in the absence of TCR stimulation. We validated the results from liver sinusoidal CD8+ T cells using intrahepatic CD8+ T cells obtained from liver tissues.Conclusions: In summary, the current study found a distinct CD56hiCD161-CD8+ T-cell population characterized by NK-like activation via TCR-independent NKG2C ligation. Further studies are required to elucidate the roles of liver sinusoidal CD56hiCD161-CD8+ T cells in immune responses to microbial pathogens or liver immunopathology.Lay summary: The role of different immune cell populations in the liver is becoming an area of increasing interest. Herein, we identified a distinct T-cell population that had features similar to those of natural killer (NK) cells - a type of innate immune cell. This distinct population was expanded in the livers of pa-tients with chronic liver disease and could thus have patho-genic relevance.(c) 2022 The Author(s). Published by Elsevier B.V. on behalf of Euro-pean Association for the Study of the Liver. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/ licenses/by-nc-nd/4.0/). | - |
dc.language | English | - |
dc.publisher | ELSEVIER | - |
dc.title | Identification of a distinct NK-like hepatic T-cell population activated by NKG2C in a TCR-independent manner | - |
dc.type | Article | - |
dc.identifier.wosid | 000868321600020 | - |
dc.identifier.scopusid | 2-s2.0-85134624263 | - |
dc.type.rims | ART | - |
dc.citation.volume | 77 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 1059 | - |
dc.citation.endingpage | 1070 | - |
dc.citation.publicationname | JOURNAL OF HEPATOLOGY | - |
dc.identifier.doi | 10.1016/j.jhep.2022.05.020 | - |
dc.contributor.localauthor | Park, Su-Hyung | - |
dc.contributor.localauthor | Shin, Eui-Cheol | - |
dc.contributor.nonIdAuthor | Choi, Seong Jin | - |
dc.contributor.nonIdAuthor | Nam, Heejin | - |
dc.contributor.nonIdAuthor | Lee, Jae Geun | - |
dc.contributor.nonIdAuthor | Kim, Myoung Soo | - |
dc.contributor.nonIdAuthor | Park, Jun Yong | - |
dc.contributor.nonIdAuthor | Joo, Dong Jin | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | CD8+T cell | - |
dc.subject.keywordAuthor | liver | - |
dc.subject.keywordAuthor | NKG2C | - |
dc.subject.keywordAuthor | NK cell receptor | - |
dc.subject.keywordAuthor | NK-like T cell | - |
dc.subject.keywordAuthor | CITE-seq | - |
dc.subject.keywordPlus | NATURAL-KILLER-CELLS | - |
dc.subject.keywordPlus | SURFACE EXPRESSION | - |
dc.subject.keywordPlus | HUMAN LIVER | - |
dc.subject.keywordPlus | IMMUNE-RESPONSES | - |
dc.subject.keywordPlus | MEMORY | - |
dc.subject.keywordPlus | IL-15 | - |
dc.subject.keywordPlus | LYMPHOCYTES | - |
dc.subject.keywordPlus | CYTOKINE | - |
dc.subject.keywordPlus | SUBSETS | - |
dc.subject.keywordPlus | INJURY | - |
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