DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chandrasekaran, Sita S. | ko |
dc.contributor.author | Agrawal, Shreeya | ko |
dc.contributor.author | Fanton, Alison | ko |
dc.contributor.author | Jangid, Aditya R. | ko |
dc.contributor.author | Charrez, Berenice | ko |
dc.contributor.author | Escajeda, Arturo M. | ko |
dc.contributor.author | Son, Sungmin | ko |
dc.contributor.author | Mcintosh, Roger | ko |
dc.contributor.author | Tran, Huyen | ko |
dc.contributor.author | Bhuiya, Abdul | ko |
dc.contributor.author | Derby, Maria Diaz de Leon | ko |
dc.contributor.author | Switz, Neil A. | ko |
dc.contributor.author | Armstrong, Maxim | ko |
dc.contributor.author | Harris, Andrew R. | ko |
dc.contributor.author | Prywes, Noam | ko |
dc.contributor.author | Lukarska, Maria | ko |
dc.contributor.author | Biering, Scott B. | ko |
dc.contributor.author | Smock, Dylan C. J. | ko |
dc.contributor.author | Mok, Amanda | ko |
dc.contributor.author | Knott, Gavin J. | ko |
dc.contributor.author | Dang, Qi | ko |
dc.contributor.author | Van, Dis Erik | ko |
dc.contributor.author | Dugan, Eli | ko |
dc.contributor.author | Kim, Shin | ko |
dc.contributor.author | Liu, Tina Y. | ko |
dc.contributor.author | Consortium, Igi Testing | ko |
dc.contributor.author | Moehle, Erica A. | ko |
dc.contributor.author | Kogut, Katherine | ko |
dc.contributor.author | Eskenazi, Brenda | ko |
dc.contributor.author | Harris, Eva | ko |
dc.contributor.author | Stanley, Sarah A. | ko |
dc.contributor.author | Lareau, Liana F. | ko |
dc.contributor.author | Tan, Ming X. | ko |
dc.contributor.author | Fletcher, Daniel A. | ko |
dc.contributor.author | Doudna, Jennifer A. | ko |
dc.contributor.author | Savage, David F. | ko |
dc.contributor.author | Hsu, Patrick D. | ko |
dc.date.accessioned | 2022-10-30T02:00:09Z | - |
dc.date.available | 2022-10-30T02:00:09Z | - |
dc.date.created | 2022-10-27 | - |
dc.date.created | 2022-10-27 | - |
dc.date.created | 2022-10-27 | - |
dc.date.issued | 2022-08 | - |
dc.identifier.citation | NATURE BIOMEDICAL ENGINEERING, v.6, no.8, pp.944 - 956 | - |
dc.identifier.issn | 2157-846X | - |
dc.identifier.uri | http://hdl.handle.net/10203/299171 | - |
dc.description.abstract | A COVID-19 test implemented in an automated microfluidic device and leveraging isothermal RNA amplification followed by T7 transcription and Cas13-mediated cleavage of a quenched fluorophore rapidly detects SARS-CoV-2 RNA in saliva samples.,Rapid nucleic acid testing is central to infectious disease surveillance. Here, we report an assay for rapid COVID-19 testing and its implementation in a prototype microfluidic device. The assay, which we named DISCoVER (for diagnostics with coronavirus enzymatic reporting), involves extraction-free sample lysis via shelf-stable and low-cost reagents, multiplexed isothermal RNA amplification followed by T7 transcription, and Cas13-mediated cleavage of a quenched fluorophore. The device consists of a single-use gravity-driven microfluidic cartridge inserted into a compact instrument for automated running of the assay and readout of fluorescence within 60 min. DISCoVER can detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in saliva with a sensitivity of 40 copies mu l(-1), and was 94% sensitive and 100% specific when validated (against quantitative PCR) using total RNA extracted from 63 nasal-swab samples (33 SARS-CoV-2-positive, with cycle-threshold values of 13-35). The device correctly identified all tested clinical saliva samples (10 SARS-CoV-2-positive out of 13, with cycle-threshold values of 23-31). Rapid point-of-care nucleic acid testing may broaden the use of molecular diagnostics., | - |
dc.language | English | - |
dc.publisher | NATURE PORTFOLIO | - |
dc.title | Rapid detection of SARS-CoV-2 RNA in saliva via Cas13 | - |
dc.type | Article | - |
dc.identifier.wosid | 000839493700002 | - |
dc.identifier.scopusid | 2-s2.0-85136023172 | - |
dc.type.rims | ART | - |
dc.citation.volume | 6 | - |
dc.citation.issue | 8 | - |
dc.citation.beginningpage | 944 | - |
dc.citation.endingpage | 956 | - |
dc.citation.publicationname | NATURE BIOMEDICAL ENGINEERING | - |
dc.identifier.doi | 10.1038/s41551-022-00917-y | - |
dc.contributor.localauthor | Son, Sungmin | - |
dc.contributor.nonIdAuthor | Chandrasekaran, Sita S. | - |
dc.contributor.nonIdAuthor | Agrawal, Shreeya | - |
dc.contributor.nonIdAuthor | Fanton, Alison | - |
dc.contributor.nonIdAuthor | Jangid, Aditya R. | - |
dc.contributor.nonIdAuthor | Charrez, Berenice | - |
dc.contributor.nonIdAuthor | Escajeda, Arturo M. | - |
dc.contributor.nonIdAuthor | Mcintosh, Roger | - |
dc.contributor.nonIdAuthor | Tran, Huyen | - |
dc.contributor.nonIdAuthor | Bhuiya, Abdul | - |
dc.contributor.nonIdAuthor | Derby, Maria Diaz de Leon | - |
dc.contributor.nonIdAuthor | Switz, Neil A. | - |
dc.contributor.nonIdAuthor | Armstrong, Maxim | - |
dc.contributor.nonIdAuthor | Harris, Andrew R. | - |
dc.contributor.nonIdAuthor | Prywes, Noam | - |
dc.contributor.nonIdAuthor | Lukarska, Maria | - |
dc.contributor.nonIdAuthor | Biering, Scott B. | - |
dc.contributor.nonIdAuthor | Smock, Dylan C. J. | - |
dc.contributor.nonIdAuthor | Mok, Amanda | - |
dc.contributor.nonIdAuthor | Knott, Gavin J. | - |
dc.contributor.nonIdAuthor | Dang, Qi | - |
dc.contributor.nonIdAuthor | Van, Dis Erik | - |
dc.contributor.nonIdAuthor | Dugan, Eli | - |
dc.contributor.nonIdAuthor | Kim, Shin | - |
dc.contributor.nonIdAuthor | Liu, Tina Y. | - |
dc.contributor.nonIdAuthor | Consortium, Igi Testing | - |
dc.contributor.nonIdAuthor | Moehle, Erica A. | - |
dc.contributor.nonIdAuthor | Kogut, Katherine | - |
dc.contributor.nonIdAuthor | Eskenazi, Brenda | - |
dc.contributor.nonIdAuthor | Harris, Eva | - |
dc.contributor.nonIdAuthor | Stanley, Sarah A. | - |
dc.contributor.nonIdAuthor | Lareau, Liana F. | - |
dc.contributor.nonIdAuthor | Tan, Ming X. | - |
dc.contributor.nonIdAuthor | Fletcher, Daniel A. | - |
dc.contributor.nonIdAuthor | Doudna, Jennifer A. | - |
dc.contributor.nonIdAuthor | Savage, David F. | - |
dc.contributor.nonIdAuthor | Hsu, Patrick D. | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | NUCLEIC-ACID DETECTION | - |
dc.subject.keywordPlus | DIAGNOSTICS | - |
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