DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Park, Hyun-Gyu | - |
dc.contributor.advisor | 박현규 | - |
dc.contributor.author | Song, Jae-Yang | - |
dc.contributor.author | 송재양 | - |
dc.date.accessioned | 2011-12-13T01:54:25Z | - |
dc.date.available | 2011-12-13T01:54:25Z | - |
dc.date.issued | 2005 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=243730&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/29860 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2005.2, [ iii, 28 p. ] | - |
dc.description.abstract | In the present study we exploited the superior features of peptide nucleic acids (PNAs) to develop an efficient PNA zip-code microarray for the detection of HNF-$\alpha$ mutations that cause type 3 maturity-onset diabetes of the young (MODY). A multi-epoxy linker compound was synthesized and used to achieve an efficient covalent linking of amine-modified PNA to an aminated glass surface. PCR was performed to amplify the genomic regions containing the mutation sites. The PCR products were then employed as templates in a subsequent multiplex SBE reaction using chimeric primers with 3´ complementarity to the specific mutation site and 5´ complementarity to the respective PNA zip-code sequence on the microarray. The primers were extended by a single base at each corresponding mutation site in the presence of biotin-labeled ddNTPs, and the products were hybridized to the PNA microarray. Compared to the corresponding DNA, the PNA zip-code sequence showed a much higher duplex specificity for the complementary DNA sequence. The PNA zip-code microarray was finally stained with streptavidin-R-phycoerythrin to generate a fluorescent signal. Using this strategy, we were able to correctly diagnose several mutation sites in exon 2 of HNF-$\alpha$ with a wild-type and mutant sample. This work represents one of the few successful applications of PNA in DNA chip technology. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | HNF-1α | - |
dc.subject | PNA zip-code microarray | - |
dc.subject | Single Base Extensionrbon dioxide | - |
dc.subject | 한 염기 확장반응이산화탄소 | - |
dc.subject | 당뇨병 | - |
dc.subject | PNA 집코드 마이크로 어레이 | - |
dc.title | Diagnosis of HNF-1α mutations on PNA zip-code microarray by single base extension | - |
dc.title.alternative | PNA 집코드 마이크로어레이를 이용한 HNF-1α 유전자 염기변이 진단 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 243730/325007 | - |
dc.description.department | 한국과학기술원 : 생명화학공학과, | - |
dc.identifier.uid | 020033341 | - |
dc.contributor.localauthor | Park, Hyun-Gyu | - |
dc.contributor.localauthor | 박현규 | - |
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