DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Ji Hun | ko |
dc.contributor.author | Rege, Mayuri | ko |
dc.contributor.author | Valeri, Jacqueline | ko |
dc.contributor.author | Dunagin, Margaret C. | ko |
dc.contributor.author | Metzger, Aryeh | ko |
dc.contributor.author | Titus, Katelyn R. | ko |
dc.contributor.author | Gilgenast, Thomas G. | ko |
dc.contributor.author | Gong, Wanfeng | ko |
dc.contributor.author | Beagan, Jonathan A. | ko |
dc.contributor.author | Raj, Arjun | ko |
dc.contributor.author | Phillips-Cremins, Jennifer E. | ko |
dc.date.accessioned | 2022-09-15T02:00:12Z | - |
dc.date.available | 2022-09-15T02:00:12Z | - |
dc.date.created | 2022-09-15 | - |
dc.date.created | 2022-09-15 | - |
dc.date.issued | 2019-07 | - |
dc.identifier.citation | NATURE METHODS, v.16, no.7, pp.633 - + | - |
dc.identifier.issn | 1548-7091 | - |
dc.identifier.uri | http://hdl.handle.net/10203/298541 | - |
dc.description.abstract | Mammalian genomes are folded into tens of thousands of long-range looping interactions. The cause-and-effect relationship between looping and genome function is poorly understood, and the extent to which loops are dynamic on short time scales remains an unanswered question. Here, we engineer a new class of synthetic architectural proteins for directed rearrangement of the three-dimensional genome using blue light. We target our light-activated-dynamic-looping (LADL) system to two genomic anchors with CRISPR guide RNAs and induce their spatial colocalization via light-induced heterodimerization of cryptochrome 2 and a dCas9-CIBN fusion protein. We apply LADL to redirect a stretch enhancer (SE) away from its endogenous Klf4 target gene and to the Zfp462 promoter. Using single-molecule RNA-FISH, we demonstrate that de novo formation of the Zfp462-SE loop correlates with a modest increase in Zfp462 expression. LADL facilitates colocalization of genomic loci without exogenous chemical cofactors and will enable future efforts to engineer reversible and oscillatory loops on short time scales. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | LADL: light-activated dynamic looping for endogenous gene expression control | - |
dc.type | Article | - |
dc.identifier.wosid | 000473098700027 | - |
dc.identifier.scopusid | 2-s2.0-85068067233 | - |
dc.type.rims | ART | - |
dc.citation.volume | 16 | - |
dc.citation.issue | 7 | - |
dc.citation.beginningpage | 633 | - |
dc.citation.endingpage | + | - |
dc.citation.publicationname | NATURE METHODS | - |
dc.identifier.doi | 10.1038/s41592-019-0436-5 | - |
dc.contributor.localauthor | Kim, Ji Hun | - |
dc.contributor.nonIdAuthor | Rege, Mayuri | - |
dc.contributor.nonIdAuthor | Valeri, Jacqueline | - |
dc.contributor.nonIdAuthor | Dunagin, Margaret C. | - |
dc.contributor.nonIdAuthor | Metzger, Aryeh | - |
dc.contributor.nonIdAuthor | Titus, Katelyn R. | - |
dc.contributor.nonIdAuthor | Gilgenast, Thomas G. | - |
dc.contributor.nonIdAuthor | Gong, Wanfeng | - |
dc.contributor.nonIdAuthor | Beagan, Jonathan A. | - |
dc.contributor.nonIdAuthor | Raj, Arjun | - |
dc.contributor.nonIdAuthor | Phillips-Cremins, Jennifer E. | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | CRYPTOCHROME 2 | - |
dc.subject.keywordPlus | GENOME | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | CTCF | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.