Effects of Transcription-Dependent Physical Perturbations on the Chromosome Dynamics in Living Cells

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Recent studies with single-particle tracking in live cells have revealed that chromatin dynamics are directly affected by transcription. However, how transcription alters the chromatin movements followed by changes in the physical properties of chromatin has not been elucidated. Here, we measured diffusion characteristics of chromatin by targeting telomeric DNA repeats with CRISPR-labeling. We found that transcription inhibitors that directly block transcription factors globally increased the movements of chromatin, while the other inhibitor that blocks transcription by DNA intercalating showed an opposite effect. We hypothesized that the increased mobility of chromatin by transcription inhibition and the decreased chromatin movement by a DNA intercalating inhibitor is due to alterations in chromatin rigidity. We also tested how volume confinement of nuclear space affects chromatin movements. We observed decreased chromatin movements under osmotic pressure and with overexpressed chromatin architectural proteins that compact chromatin.
Publisher
FRONTIERS MEDIA SA
Issue Date
2022-07
Language
English
Article Type
Article
Citation

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, v.10

ISSN
2296-634X
DOI
10.3389/fcell.2022.822026
URI
http://hdl.handle.net/10203/297678
Appears in Collection
BS-Journal Papers(저널논문)
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