DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yim, Sung Sun | ko |
dc.contributor.author | Johns, Nathan, I | ko |
dc.contributor.author | Park, Jimin | ko |
dc.contributor.author | Gomes, Antonio L. C. | ko |
dc.contributor.author | McBee, Ross M. | ko |
dc.contributor.author | Richardson, Miles | ko |
dc.contributor.author | Ronda, Carlotta | ko |
dc.contributor.author | Chen, Sway P. | ko |
dc.contributor.author | Garenne, David | ko |
dc.contributor.author | Noireaux, Vincent | ko |
dc.contributor.author | Wang, Harris H. | ko |
dc.date.accessioned | 2022-07-20T02:00:26Z | - |
dc.date.available | 2022-07-20T02:00:26Z | - |
dc.date.created | 2022-07-20 | - |
dc.date.issued | 2019-08 | - |
dc.identifier.citation | MOLECULAR SYSTEMS BIOLOGY, v.15, no.8 | - |
dc.identifier.issn | 1744-4292 | - |
dc.identifier.uri | http://hdl.handle.net/10203/297408 | - |
dc.description.abstract | Cell-free expression systems enable rapid prototyping of genetic programs in vitro. However, current throughput of cell-free measurements is limited by the use of channel-limited fluorescent readouts. Here, we describe DNA Regulatory element Analysis by cell-Free Transcription and Sequencing (DRAFTS), a rapid and robust in vitro approach for multiplexed measurement of transcriptional activities from thousands of regulatory sequences in a single reaction. We employ this method in active cell lysates developed from ten diverse bacterial species. Interspecies analysis of transcriptional profiles from > 1,000 diverse regulatory sequences reveals functional differences in promoter activity that can be quantitatively modeled, providing a rich resource for tuning gene expression in diverse bacterial species. Finally, we examine the transcriptional capacities of dual-species hybrid lysates that can simultaneously harness gene expression properties of multiple organisms. We expect that this cell-free multiplex transcriptional measurement approach will improve genetic part prototyping in new bacterial chassis for synthetic biology. | - |
dc.language | English | - |
dc.publisher | WILEY | - |
dc.title | Multiplex transcriptional characterizations across diverse bacterial species using cell-free systems | - |
dc.type | Article | - |
dc.identifier.wosid | 000484419200006 | - |
dc.identifier.scopusid | 2-s2.0-85071436541 | - |
dc.type.rims | ART | - |
dc.citation.volume | 15 | - |
dc.citation.issue | 8 | - |
dc.citation.publicationname | MOLECULAR SYSTEMS BIOLOGY | - |
dc.identifier.doi | 10.15252/msb.20198875 | - |
dc.contributor.localauthor | Yim, Sung Sun | - |
dc.contributor.nonIdAuthor | Johns, Nathan, I | - |
dc.contributor.nonIdAuthor | Park, Jimin | - |
dc.contributor.nonIdAuthor | Gomes, Antonio L. C. | - |
dc.contributor.nonIdAuthor | McBee, Ross M. | - |
dc.contributor.nonIdAuthor | Richardson, Miles | - |
dc.contributor.nonIdAuthor | Ronda, Carlotta | - |
dc.contributor.nonIdAuthor | Chen, Sway P. | - |
dc.contributor.nonIdAuthor | Garenne, David | - |
dc.contributor.nonIdAuthor | Noireaux, Vincent | - |
dc.contributor.nonIdAuthor | Wang, Harris H. | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | cell-free expression systems | - |
dc.subject.keywordAuthor | gene expression | - |
dc.subject.keywordAuthor | massively parallel reporter assay | - |
dc.subject.keywordAuthor | synthetic biology | - |
dc.subject.keywordAuthor | transcription | - |
dc.subject.keywordPlus | FREE PROTEIN-SYNTHESIS | - |
dc.subject.keywordPlus | DNA REGULATORY ELEMENTS | - |
dc.subject.keywordPlus | TRANSLATION SYSTEM | - |
dc.subject.keywordPlus | SYNTHETIC BIOLOGY | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordPlus | PRINCIPLES | - |
dc.subject.keywordPlus | EVOLUTION | - |
dc.subject.keywordPlus | SELECTION | - |
dc.subject.keywordPlus | PLATFORM | - |
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