Multiplex transcriptional characterizations across diverse bacterial species using cell-free systems

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dc.contributor.authorYim, Sung Sunko
dc.contributor.authorJohns, Nathan, Iko
dc.contributor.authorPark, Jiminko
dc.contributor.authorGomes, Antonio L. C.ko
dc.contributor.authorMcBee, Ross M.ko
dc.contributor.authorRichardson, Milesko
dc.contributor.authorRonda, Carlottako
dc.contributor.authorChen, Sway P.ko
dc.contributor.authorGarenne, Davidko
dc.contributor.authorNoireaux, Vincentko
dc.contributor.authorWang, Harris H.ko
dc.date.accessioned2022-07-20T02:00:26Z-
dc.date.available2022-07-20T02:00:26Z-
dc.date.created2022-07-20-
dc.date.issued2019-08-
dc.identifier.citationMOLECULAR SYSTEMS BIOLOGY, v.15, no.8-
dc.identifier.issn1744-4292-
dc.identifier.urihttp://hdl.handle.net/10203/297408-
dc.description.abstractCell-free expression systems enable rapid prototyping of genetic programs in vitro. However, current throughput of cell-free measurements is limited by the use of channel-limited fluorescent readouts. Here, we describe DNA Regulatory element Analysis by cell-Free Transcription and Sequencing (DRAFTS), a rapid and robust in vitro approach for multiplexed measurement of transcriptional activities from thousands of regulatory sequences in a single reaction. We employ this method in active cell lysates developed from ten diverse bacterial species. Interspecies analysis of transcriptional profiles from > 1,000 diverse regulatory sequences reveals functional differences in promoter activity that can be quantitatively modeled, providing a rich resource for tuning gene expression in diverse bacterial species. Finally, we examine the transcriptional capacities of dual-species hybrid lysates that can simultaneously harness gene expression properties of multiple organisms. We expect that this cell-free multiplex transcriptional measurement approach will improve genetic part prototyping in new bacterial chassis for synthetic biology.-
dc.languageEnglish-
dc.publisherWILEY-
dc.titleMultiplex transcriptional characterizations across diverse bacterial species using cell-free systems-
dc.typeArticle-
dc.identifier.wosid000484419200006-
dc.identifier.scopusid2-s2.0-85071436541-
dc.type.rimsART-
dc.citation.volume15-
dc.citation.issue8-
dc.citation.publicationnameMOLECULAR SYSTEMS BIOLOGY-
dc.identifier.doi10.15252/msb.20198875-
dc.contributor.localauthorYim, Sung Sun-
dc.contributor.nonIdAuthorJohns, Nathan, I-
dc.contributor.nonIdAuthorPark, Jimin-
dc.contributor.nonIdAuthorGomes, Antonio L. C.-
dc.contributor.nonIdAuthorMcBee, Ross M.-
dc.contributor.nonIdAuthorRichardson, Miles-
dc.contributor.nonIdAuthorRonda, Carlotta-
dc.contributor.nonIdAuthorChen, Sway P.-
dc.contributor.nonIdAuthorGarenne, David-
dc.contributor.nonIdAuthorNoireaux, Vincent-
dc.contributor.nonIdAuthorWang, Harris H.-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorcell-free expression systems-
dc.subject.keywordAuthorgene expression-
dc.subject.keywordAuthormassively parallel reporter assay-
dc.subject.keywordAuthorsynthetic biology-
dc.subject.keywordAuthortranscription-
dc.subject.keywordPlusFREE PROTEIN-SYNTHESIS-
dc.subject.keywordPlusDNA REGULATORY ELEMENTS-
dc.subject.keywordPlusTRANSLATION SYSTEM-
dc.subject.keywordPlusSYNTHETIC BIOLOGY-
dc.subject.keywordPlusRNA-
dc.subject.keywordPlusDESIGN-
dc.subject.keywordPlusPRINCIPLES-
dc.subject.keywordPlusEVOLUTION-
dc.subject.keywordPlusSELECTION-
dc.subject.keywordPlusPLATFORM-
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