DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yim, Sung Sun | ko |
dc.contributor.author | Johns, Nathan, I | ko |
dc.contributor.author | Noireaux, Vincent | ko |
dc.contributor.author | Wang, Harris H. | ko |
dc.date.accessioned | 2022-07-20T02:00:23Z | - |
dc.date.available | 2022-07-20T02:00:23Z | - |
dc.date.created | 2022-07-20 | - |
dc.date.issued | 2020-10 | - |
dc.identifier.citation | ACS SYNTHETIC BIOLOGY, v.9, no.10, pp.2851 - 2855 | - |
dc.identifier.issn | 2161-5063 | - |
dc.identifier.uri | http://hdl.handle.net/10203/297407 | - |
dc.description.abstract | Recent advances in cell-free systems have opened up new capabilities in synthetic biology from rapid prototyping of genetic circuits and metabolic pathways to portable diagnostics and biomanufacturing. A current bottleneck in cell-free systems, especially those employing non-E. coli bacterial species, is the required use of plasmid DNA, which can be laborious to construct, clone, and verify. Linear DNA templates offer a faster and more direct route for many cell-free applications, but they are often rapidly degraded in cell-free reactions. In this study, we evaluated GamS from lambda-phage, DNA fragments containing Chi-sites, and Ku from Mycobacterium tuberculosis for their ability to protect linear DNA templates in diverse bacterial cell-free systems. We show that these nuclease inhibitors exhibit differential protective activities against endogenous exonucleases in five different cell-free lysates, highlighting their utility for diverse bacterial species. We expect these linear DNA protection strategies will accelerate high-throughput approaches in cell-free synthetic biology. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Protecting Linear DNA Templates in Cell-Free Expression Systems from Diverse Bacteria | - |
dc.type | Article | - |
dc.identifier.wosid | 000582582100023 | - |
dc.identifier.scopusid | 2-s2.0-85096590035 | - |
dc.type.rims | ART | - |
dc.citation.volume | 9 | - |
dc.citation.issue | 10 | - |
dc.citation.beginningpage | 2851 | - |
dc.citation.endingpage | 2855 | - |
dc.citation.publicationname | ACS SYNTHETIC BIOLOGY | - |
dc.identifier.doi | 10.1021/acssynbio.0c00277 | - |
dc.contributor.localauthor | Yim, Sung Sun | - |
dc.contributor.nonIdAuthor | Johns, Nathan, I | - |
dc.contributor.nonIdAuthor | Noireaux, Vincent | - |
dc.contributor.nonIdAuthor | Wang, Harris H. | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | cell-free expression systems | - |
dc.subject.keywordAuthor | linear DNA | - |
dc.subject.keywordAuthor | RecBCD | - |
dc.subject.keywordAuthor | GamS | - |
dc.subject.keywordAuthor | Chi | - |
dc.subject.keywordAuthor | Ku | - |
dc.subject.keywordPlus | REPAIR | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | STABILITY | - |
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