(The) effects of transport of actinomycin D across egg phosphatidylcholine lipid bilayerEgg PC 이중층을 통한 항암제actinomycin D의 투과 특성에 대한 영향

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dc.contributor.advisorKim, Jong-Duck-
dc.contributor.advisor김종득-
dc.contributor.authorKim, Se-Hwa-
dc.contributor.author김세화-
dc.date.accessioned2011-12-13T01:52:11Z-
dc.date.available2011-12-13T01:52:11Z-
dc.date.issued2001-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=166016&flag=dissertation-
dc.identifier.urihttp://hdl.handle.net/10203/29716-
dc.description학위논문(석사) - 한국과학기술원 : 화학공학과, 2001.2, [ xi, 70 p. ]-
dc.description.abstractActinomycin D(AMD), a potent antitumor agent, was incorporated into egg phosphatidylcholine vesicles mixed with various molar ratio of cholesterol, to yield information about effective loading method. Also, the influences of cholesterol and temperature on the molecular transport of actinomycin D across large unilamellar lipid bilayer were studied. The two types of loading methods for liposomal formulation of AMD were introduced and each efficacy was investigated in terms of entrapment efficiency and stability of drug. In physical binding method, the drug formed complexes with lipid mixtures and the entrapment efficiency was reduced with the addition of cholesterol because AMD served as membrane constituents. In transmembrane loading method, the drug transported through the bilayer by pH gradient with high entrapment efficiency. The size distribution of liposome suspensions was not significantly affected by the addition of cholesterol in transmembrane loading method, whereas in the physical binding method, the increase of effective diameter was observed, originated from the aggregates of cholesterol. In the natural release experiment, the liposome formulation was more stable when prepared by the physical binding method than that of the transmembrane loading method was. In the transmembrane loading method, the effect of transbilayer pH gradient was studied on the rate of uptake and entrapment efficiency. Liposomes prepared with a transbilayer pH gradient of 1.4 units (inner pH is 7.4, outer pH is 8.8), showed the higher entrapment efficiency and the faster rates of translocation of drug than vesicles without pH gradient did. In order to increase the rate of uptake, various temperatures of loading were employed. A First order kinetic analysis was employed to determine the activation energy and to compare the rates of uptake quantitatively. The activation energy was 2.1 fold larger in liposomes without ΔpH than that of vesicles with ΔpH 1.4. The effects of ch...eng
dc.languageeng-
dc.publisher한국과학기술원-
dc.subjectfluidity of lipid bilayer-
dc.subjecttemperature-
dc.subjectcholesterol-
dc.subjectEgg PC bilayer-
dc.subjectActinomycin D-
dc.subjectActinomycin D-
dc.subject지질 이중막 유동성-
dc.subject온도-
dc.subject콜레스테롤-
dc.subjectEgg PC 이중층-
dc.title(The) effects of transport of actinomycin D across egg phosphatidylcholine lipid bilayer-
dc.title.alternativeEgg PC 이중층을 통한 항암제actinomycin D의 투과 특성에 대한 영향-
dc.typeThesis(Master)-
dc.identifier.CNRN166016/325007-
dc.description.department한국과학기술원 : 화학공학과, -
dc.identifier.uid000993105-
dc.contributor.localauthorKim, Jong-Duck-
dc.contributor.localauthor김종득-
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