DC Field | Value | Language |
---|---|---|
dc.contributor.author | Nayak, Manoranjan | ko |
dc.contributor.author | Suh, William | ko |
dc.contributor.author | Oh, Young Taek | ko |
dc.contributor.author | Ryu, Ae Jin | ko |
dc.contributor.author | Jeong, Ki Jun | ko |
dc.contributor.author | Kim, Minsik | ko |
dc.contributor.author | Mohapatra, Ranjan Kumar | ko |
dc.contributor.author | Lee, Bongsoo | ko |
dc.contributor.author | Chang, Yong Keun | ko |
dc.date.accessioned | 2022-05-06T05:00:40Z | - |
dc.date.available | 2022-05-06T05:00:40Z | - |
dc.date.created | 2022-05-06 | - |
dc.date.created | 2022-05-06 | - |
dc.date.issued | 2022-05 | - |
dc.identifier.citation | FUEL, v.316 | - |
dc.identifier.issn | 0016-2361 | - |
dc.identifier.uri | http://hdl.handle.net/10203/296356 | - |
dc.description.abstract | The present study aimed to improve the lipid content and lipid productivity of Chlorella sp. HS2 by exposing them to ethyl methanesulfonate (EMS) to generate a mutant and followed with fluorescent assorted cell sorting (FACS) for the selection of mutant cells having desirable phenotypes. The microalgae were subjected under 100 mu mol mL(-1) concentration of EMS for 30 min, followed by intracellular lipid staining with boron-dipyrromethene (BODIPY) fluorophore for FACS based selection. The biomass growth, lipid content, lipid productivity, and fatty acid composition of the selected mutagenized strain (SMS, TE+F) of Chlorella sp. HS2 were compared against those of FACS selected strain without mutagenesis (FSS, T-F) and parent wild strain (PWS, TC), under two different carbon supplementation schemes (only CO2 supply and optimized carbon supply). The highest lipid content and productivity of 35.6% and 248.7 mg L-1 d-(1), respectively were observed in the SMS grown under optimized carbon supplementation (1% CO2 (v/v) and 0.5 g L-1 NaHCO3), which was a substantial improvement over those of FSS (26.6% and 193.4 mg L(-1 )d(-1)) and PWS (24.7% and 153.6 mg L-1 d(-1)). The fatty acid methyl ester (FAME) profile of the lipids derived from SMS showed a marked increase in the proportion of saturated fatty acids (SFA) and mono-unsaturated fatty acids (MUFA) (76.7%) over those of FSS (70.2%) and PWS (69.6%). Lastly, fuel properties were found to be appropriate for biodiesel production, as they corroborate with international fuel standards. These results indicate that directed evolution using EMS mutagenesis with FACS is a powerful tool to achieve microalgal strain improvement for biofuel production. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Directed evolution of Chlorella sp. HS2 towards enhanced lipid accumulation by ethyl methanesulfonate mutagenesis in conjunction with fluorescence-activated cell sorting based screening | - |
dc.type | Article | - |
dc.identifier.wosid | 000783173000010 | - |
dc.identifier.scopusid | 2-s2.0-85123592618 | - |
dc.type.rims | ART | - |
dc.citation.volume | 316 | - |
dc.citation.publicationname | FUEL | - |
dc.identifier.doi | 10.1016/j.fuel.2022.123410 | - |
dc.contributor.localauthor | Jeong, Ki Jun | - |
dc.contributor.localauthor | Chang, Yong Keun | - |
dc.contributor.nonIdAuthor | Nayak, Manoranjan | - |
dc.contributor.nonIdAuthor | Suh, William | - |
dc.contributor.nonIdAuthor | Oh, Young Taek | - |
dc.contributor.nonIdAuthor | Mohapatra, Ranjan Kumar | - |
dc.contributor.nonIdAuthor | Lee, Bongsoo | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Chlorella sp | - |
dc.subject.keywordAuthor | HS2 | - |
dc.subject.keywordAuthor | EMS | - |
dc.subject.keywordAuthor | FACS | - |
dc.subject.keywordAuthor | Lipid productivity | - |
dc.subject.keywordAuthor | FAME | - |
dc.subject.keywordAuthor | Biodiesel | - |
dc.subject.keywordPlus | PRODUCTIVITY | - |
dc.subject.keywordPlus | MICROALGAE | - |
dc.subject.keywordPlus | BICARBONATE | - |
dc.subject.keywordPlus | BIOMASS | - |
dc.subject.keywordPlus | EMS | - |
dc.subject.keywordPlus | SUPPLEMENTATION | - |
dc.subject.keywordPlus | BIODIESEL | - |
dc.subject.keywordPlus | VULGARIS | - |
dc.subject.keywordPlus | PROFILE | - |
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