DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Yoon, Young-Gyu | - |
dc.contributor.advisor | 윤영규 | - |
dc.contributor.author | Cho, Eun-Seo | - |
dc.date.accessioned | 2022-04-27T19:31:27Z | - |
dc.date.available | 2022-04-27T19:31:27Z | - |
dc.date.issued | 2021 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=948981&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/296026 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 전기및전자공학부, 2021.2,[iii, 27 p. :] | - |
dc.description.abstract | Understanding underlying principles of neural circuits is a central goal in neuroscience. A high-speed 3-D imaging system is required to monitor the neuronal activity of whole-brain in order to study internal brain dynamics. Here we propose Sparse Decomposition Deconvolution Microscopy (SDM), a computational microscopy method for volumetric imaging of neuronal activity. The work in this thesis is composed of two parts. The first part of the thesis describes the development of SDM and the second part describes the demonstration of its capability in larval zebrafish expressing pan-neuronal GCaMP7a. SDM overcomes the major challenge of deconvolution microscopy, that 3-D deconvolution of wide-field microscopy images is an ill-posed inverse problem, by reformulating the inverse problem and applying sparse decomposition prior to deconvolution. We demonstrate the capability of SDM via in vivo imaging of neuronal activity of the whole-brain of the larval zebrafish with a volumetric field of view of 780μm×438μm×200μm at imaging rate up to 1−4.98 volumes per second. We expect the proposed microscopy to be used as an attractive tool for high-speed 3-D calcium imaging. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Sparse decomposition▼aDeconvolution microscopy▼aHigh-speed 3-D imaging system▼aElectrically tunable lens▼aWhole-brain imaging▼aCalcium imaging▼aZebrafish | - |
dc.subject | 희소분해▼a디컨볼루션 현미경법▼a고속 3-D 이미징 시스템▼a전기로 조절 가능한 렌즈▼a뇌 전체 이미징▼a칼슘 이미징▼a제브라피쉬 | - |
dc.title | Sparse decomposition deconvolution microscopy for high-speed 3-D imaging of neuronal activity | - |
dc.title.alternative | 신경활동의 고속 3차원 이미징을 위한 희소분해 디컨볼루션 현미경법 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :전기및전자공학부, | - |
dc.contributor.alternativeauthor | 조은서 | - |
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