DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Park, Hee-Sung | - |
dc.contributor.advisor | 박희성 | - |
dc.contributor.author | Gang, Donghyeok | - |
dc.date.accessioned | 2022-04-21T19:34:54Z | - |
dc.date.available | 2022-04-21T19:34:54Z | - |
dc.date.issued | 2021 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=962519&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/295803 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 화학과, 2021.8,[ix, 119 p. :] | - |
dc.description.abstract | Depending on sizes, shapes and the kinds of surface stabilizing ligand, gold nanoparticles shows various physical and chemical properties. Especially, it’s call as ‘ultra-small nanoparticle’ if the diameter of the nanoparticle is less than 3 nm and the physical-, chemical properties of ultra-small nanoparticle is obviously different from that of larger size nanoparticles. The attractive properties (small size, high stability, chemoselectivity and ease of detection under transmission electron microscopy) suggest that usage of ultra-small gold nanoparticle in area of cryo-electron microscopy such as single-particle analysis for biomolecule structure determination and cell tomography is highly feasible. In this thesis, the repeatable synthetic method of ultra-small gold nanoparticle was screened and established. Also, for the usage of the nanoparticle in analysis of biomaterials (proteins or cells) using cryo-electrom microscopy, bio-orthogonal formation of covalent conjugation between ultra-small gold nanoparticle and protein which site-specifically non-canonical amino acid incorporated was studied. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Gold nanoparticle▼aUltra-small▼aNon-canonical amino acid▼aCryo-electron microscopy▼aProtein | - |
dc.subject | 금 나노 입자▼a초미세▼a비천연 아미노산▼a초저온 전자현미경▼a단백질 | - |
dc.title | Site-selective protein labeling with ultra-small gold nanoparticle using bio-orthogonal chemistry | - |
dc.title.alternative | 생직교화학을 이용한 초미세 금 나노입자의 단백질 위치-선택적 표지 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :화학과, | - |
dc.contributor.alternativeauthor | 강동혁 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.