DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Oh, Byung-Ha | - |
dc.contributor.advisor | 오병하 | - |
dc.contributor.author | Noh, Haemin | - |
dc.date.accessioned | 2022-04-15T01:54:10Z | - |
dc.date.available | 2022-04-15T01:54:10Z | - |
dc.date.issued | 2021 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=956431&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/294621 | - |
dc.description.abstract | Hypothetical protein is a protein which is translated from the open reading frame but its biological function or mechanism is unknown. Herein, we report the crystal structure of one of the hypothetical proteins, PYCH_01220 in Pyrococcus yayanosii CH1. This protein is composed of two domains, named Domain A and Domain B. While Domain B is not significantly homologous to any of known protein structures, Domain A is structurally analogous to the C-terminal ribonuclease domain of Escherichia coli colicin D. Domain A has a positively charged surface patch rendered by 13 basic residues, eight arginine or lysine residues of which are evolutionarily conserved. Electrophoretic mobility shift assays showed that PYCH_01220 binds to DNA, and charge-inversion mutations on this patch negatively affect the DNA binding, suggesting that the function of PYCH_01220 might involve nucleic acid-binding via the positively charged patch. | - |
dc.language | eng | - |
dc.title | Structural and biochemical studies on a hypothetical protein PYCH_01220 from Pyrococcus yayanosii | - |
dc.title.alternative | Pyrococcus yayanosii 고세균의 未同定 단백질인 PYCH_01220 구조 및 생화학적 연구 | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :생명과학과, | - |
dc.description.isOpenAccess | 학위논문(박사) - 한국과학기술원 : 생명과학과, 2021.2,[iv, 71 p. :] | - |
dc.publisher.country | 한국과학기술원 | - |
dc.type.journalArticle | Thesis(Ph.D) | - |
dc.contributor.alternativeauthor | 노해민 | - |
dc.subject.keywordAuthor | Hypothetical protein▼aPYCH_01220▼aX-ray crystallography▼aDNA-binding protein▼aColicin D | - |
dc.subject.keywordAuthor | 가설 단백질▼aPYCH_01220▼a엑스선 결정학▼aDNA 결합 단백질▼aColicin D | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.