DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Tae-Shin | ko |
dc.contributor.author | Rha, Min-Seok | ko |
dc.contributor.author | Shin, Eui-Cheol | ko |
dc.date.accessioned | 2022-02-27T06:42:31Z | - |
dc.date.available | 2022-02-27T06:42:31Z | - |
dc.date.created | 2022-02-27 | - |
dc.date.created | 2022-02-27 | - |
dc.date.created | 2022-02-27 | - |
dc.date.issued | 2022-01 | - |
dc.identifier.citation | JOURNAL OF IMMUNOLOGY, v.208, no.2, pp.338 - 346 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.uri | http://hdl.handle.net/10203/292444 | - |
dc.description.abstract | IL-15 exhibits pleiotropic effects on NK and CD8(+) T cells and contributes to host protection or immunopathology during infection. Although both type I IFNs and IFN-gamma upregulate IL-15 expression, their effects on IL-15 upregulation and underlying mechanisms have not been compared comprehensively. In addition, little is known about trans-presentation of IL-15 by epithelial cells to lymphocytes. In this study, we analyzed the expression of IL-15 and IL-15R alpha in the human hepatocyte-derived Huh-7 cell line after stimulation with IFN-alpha, IFN-beta, or IFN-gamma using RT-PCR, flow cytometry, and confocal microscopy. We also performed knockdown experiments to investigate the signaling pathway involved in IL-15 upregulation. IFN-gamma more potently upregulated IL-15 expression in Huh-7 cells than IFN-alpha and IFN-beta. Knockdown experiments revealed that IFN-gamma- and IFN-beta-induced IL-15 expression relied on IFN regulatory factor 1 (IRF1), which is upregulated by STAT1 and IFN-stimulated gene factor 3, respectively. Inhibitor of kappa B kinase alpha/beta was also involved in IFN-gamma-induced upregulation of IL-15. Furthermore, human NK cells were activated by coculture with IFN-gamma-treated Huh-7 cells, which was abrogated by knocking down IL-15R alpha in IFN-gamma-treated Huh-7 cells, indicating that IFN-gamma-induced IL-15 on Huh-7 cells activates NK cells via trans-presentation. In summary, our data demonstrate that IFN-gamma potently elicits IL-15 trans-presentation by epithelial cells via IRF1. These data also suggest that the IFN gamma-IRF1-IL-15 axis may be a regulatory target for the treatment of diseases with IL-15 dysregulation. | - |
dc.language | English | - |
dc.publisher | AMER ASSOC IMMUNOLOGISTS | - |
dc.title | IFN-gamma Induces IL-15 Trans-Presentation by Epithelial Cells via IRF1 | - |
dc.type | Article | - |
dc.identifier.wosid | 000752651600018 | - |
dc.identifier.scopusid | 2-s2.0-85123651868 | - |
dc.type.rims | ART | - |
dc.citation.volume | 208 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 338 | - |
dc.citation.endingpage | 346 | - |
dc.citation.publicationname | JOURNAL OF IMMUNOLOGY | - |
dc.identifier.doi | 10.4049/jimmunol.2100057 | - |
dc.contributor.localauthor | Shin, Eui-Cheol | - |
dc.contributor.nonIdAuthor | Kim, Tae-Shin | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | CD8(+) T | - |
dc.subject.keywordPlus | DENDRITIC CELLS | - |
dc.subject.keywordPlus | HUMAN MONOCYTES | - |
dc.subject.keywordPlus | KILLER-CELLS | - |
dc.subject.keywordPlus | NK CELLS | - |
dc.subject.keywordPlus | INTERLEUKIN-15 | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | IL-15R-ALPHA | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | ACTIVATE | - |
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