IFN-gamma Induces IL-15 Trans-Presentation by Epithelial Cells via IRF1

Cited 5 time in webofscience Cited 0 time in scopus
  • Hit : 1827
  • Download : 0
DC FieldValueLanguage
dc.contributor.authorKim, Tae-Shinko
dc.contributor.authorRha, Min-Seokko
dc.contributor.authorShin, Eui-Cheolko
dc.date.accessioned2022-02-27T06:42:31Z-
dc.date.available2022-02-27T06:42:31Z-
dc.date.created2022-02-27-
dc.date.created2022-02-27-
dc.date.created2022-02-27-
dc.date.issued2022-01-
dc.identifier.citationJOURNAL OF IMMUNOLOGY, v.208, no.2, pp.338 - 346-
dc.identifier.issn0022-1767-
dc.identifier.urihttp://hdl.handle.net/10203/292444-
dc.description.abstractIL-15 exhibits pleiotropic effects on NK and CD8(+) T cells and contributes to host protection or immunopathology during infection. Although both type I IFNs and IFN-gamma upregulate IL-15 expression, their effects on IL-15 upregulation and underlying mechanisms have not been compared comprehensively. In addition, little is known about trans-presentation of IL-15 by epithelial cells to lymphocytes. In this study, we analyzed the expression of IL-15 and IL-15R alpha in the human hepatocyte-derived Huh-7 cell line after stimulation with IFN-alpha, IFN-beta, or IFN-gamma using RT-PCR, flow cytometry, and confocal microscopy. We also performed knockdown experiments to investigate the signaling pathway involved in IL-15 upregulation. IFN-gamma more potently upregulated IL-15 expression in Huh-7 cells than IFN-alpha and IFN-beta. Knockdown experiments revealed that IFN-gamma- and IFN-beta-induced IL-15 expression relied on IFN regulatory factor 1 (IRF1), which is upregulated by STAT1 and IFN-stimulated gene factor 3, respectively. Inhibitor of kappa B kinase alpha/beta was also involved in IFN-gamma-induced upregulation of IL-15. Furthermore, human NK cells were activated by coculture with IFN-gamma-treated Huh-7 cells, which was abrogated by knocking down IL-15R alpha in IFN-gamma-treated Huh-7 cells, indicating that IFN-gamma-induced IL-15 on Huh-7 cells activates NK cells via trans-presentation. In summary, our data demonstrate that IFN-gamma potently elicits IL-15 trans-presentation by epithelial cells via IRF1. These data also suggest that the IFN gamma-IRF1-IL-15 axis may be a regulatory target for the treatment of diseases with IL-15 dysregulation.-
dc.languageEnglish-
dc.publisherAMER ASSOC IMMUNOLOGISTS-
dc.titleIFN-gamma Induces IL-15 Trans-Presentation by Epithelial Cells via IRF1-
dc.typeArticle-
dc.identifier.wosid000752651600018-
dc.identifier.scopusid2-s2.0-85123651868-
dc.type.rimsART-
dc.citation.volume208-
dc.citation.issue2-
dc.citation.beginningpage338-
dc.citation.endingpage346-
dc.citation.publicationnameJOURNAL OF IMMUNOLOGY-
dc.identifier.doi10.4049/jimmunol.2100057-
dc.contributor.localauthorShin, Eui-Cheol-
dc.contributor.nonIdAuthorKim, Tae-Shin-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordPlusCD8(+) T-
dc.subject.keywordPlusDENDRITIC CELLS-
dc.subject.keywordPlusHUMAN MONOCYTES-
dc.subject.keywordPlusKILLER-CELLS-
dc.subject.keywordPlusNK CELLS-
dc.subject.keywordPlusINTERLEUKIN-15-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusIL-15R-ALPHA-
dc.subject.keywordPlusINDUCTION-
dc.subject.keywordPlusACTIVATE-
Appears in Collection
MSE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 5 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0