Visualization of chromatin higher-order structures and dynamics in live cells

Cited 4 time in webofscience Cited 0 time in scopus
  • Hit : 165
  • Download : 131
Chromatin has highly organized structures in the nucleus, and these higher-order structures are proposed to regulate gene activities and cellular processes. Sequencing-based techniques, such as Hi-C, and fluorescent in situ hybridization (FISH) have revealed a spatial segregation of active and inactive compartments of chromatin, as well as the non-random positioning of chromosomes in the nucleus, respectively. However, regardless of their efficiency in capturing target genomic sites, these techniques are limited to fixed cells. Since chromatin has dynamic structures, live cell imaging techniques are highlighted for their ability to detect conformational changes in chromatin at a specific time point, or to track various arrangements of chromatin through long-term imaging. Given that the imaging approaches to study live cells are dramatically advanced, we recapitulate methods that are widely used to visualize the dynamics of higher-order chromatin structures.
Publisher
KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
Issue Date
2021-10
Language
English
Article Type
Review
Citation

BMB REPORTS, v.54, no.10, pp.489 - 496

ISSN
1976-6696
DOI
10.5483/BMBRep.2021.54.10.098
URI
http://hdl.handle.net/10203/289376
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
122427.pdf(2.92 MB)Download
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 4 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0