DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ju, Yong | ko |
dc.contributor.author | Kim, Jaemin | ko |
dc.contributor.author | Park, Yeonkyung | ko |
dc.contributor.author | Lee, Chang Yeol | ko |
dc.contributor.author | Kim, Kyungnam | ko |
dc.contributor.author | Hong, Ki Ho | ko |
dc.contributor.author | Lee, Hyukmin | ko |
dc.contributor.author | Yong, Dongeun | ko |
dc.contributor.author | Park, Hyun Gyu | ko |
dc.date.accessioned | 2021-11-09T06:40:56Z | - |
dc.date.available | 2021-11-09T06:40:56Z | - |
dc.date.created | 2021-11-09 | - |
dc.date.created | 2021-11-09 | - |
dc.date.created | 2021-11-09 | - |
dc.date.created | 2021-11-09 | - |
dc.date.issued | 2022-01 | - |
dc.identifier.citation | BIOSENSORS & BIOELECTRONICS, v.196, pp.113689 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | http://hdl.handle.net/10203/288952 | - |
dc.description.abstract | We herein describe rapid and accurate clinical testing for COVID-19 by nicking and extension chain reaction system-based amplification (NESBA), an ultrasensitive version of NASBA. The primers to identify SARS-CoV-2 viral RNA were designed to additionally contain the nicking recognition sequence at the 5 '-end of conventional NASBA primers, which would enable nicking enzyme-aided exponential amplification of T7 RNA promoter-containing double-stranded DNA (T7DNA). As a consequence of this substantially enhanced amplification power, the NESBA technique was able to ultrasensitively detect SARS-CoV-2 genomic RNA (gRNA) down to 0.5 copies/mu L (= 10 copies/reaction) for both envelope (E) and nucleocapsid (N) genes within 30 min under isothermal temperature (41 degrees C). When the NESBA was applied to test a large cohort of clinical samples (n = 98), the results fully agreed with those from qRT-PCR and showed the excellent accuracy by yielding 100% clinical sensitivity and specificity. By employing multiple molecular beacons with different fluorophore labels, the NESBA was further modulated to achieve multiplex molecular diagnostics, so that the E and N genes of SARSCoV-2 gRNA were simultaneously assayed in one-pot. By offering the superior analytical performances over the current qRT-PCR, the isothermal NESBA technique could serve as very powerful platform technology to realize the point-of-care (POC) diagnosis for COVID-19. | - |
dc.language | English | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | Rapid and accurate clinical testing for COVID-19 by nicking and extension chain reaction system-based amplification (NESBA) | - |
dc.type | Article | - |
dc.identifier.wosid | 000711431100008 | - |
dc.identifier.scopusid | 2-s2.0-85117357194 | - |
dc.type.rims | ART | - |
dc.citation.volume | 196 | - |
dc.citation.beginningpage | 113689 | - |
dc.citation.publicationname | BIOSENSORS & BIOELECTRONICS | - |
dc.identifier.doi | 10.1016/j.bios.2021.113689 | - |
dc.contributor.localauthor | Park, Hyun Gyu | - |
dc.contributor.nonIdAuthor | Kim, Kyungnam | - |
dc.contributor.nonIdAuthor | Hong, Ki Ho | - |
dc.contributor.nonIdAuthor | Lee, Hyukmin | - |
dc.contributor.nonIdAuthor | Yong, Dongeun | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | COVID-19 | - |
dc.subject.keywordAuthor | SARS-CoV-2 | - |
dc.subject.keywordAuthor | qRT-PCR | - |
dc.subject.keywordAuthor | Isothermal amplification | - |
dc.subject.keywordAuthor | NESBA | - |
dc.subject.keywordAuthor | NASBA | - |
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