DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kaake, Robyn M. | ko |
dc.contributor.author | Echeverria, Ignacia | ko |
dc.contributor.author | Kim, Seung Joong | ko |
dc.contributor.author | Von Dollen, John | ko |
dc.contributor.author | Chesarino, Nicholas M. | ko |
dc.contributor.author | Feng, Yuqing | ko |
dc.contributor.author | Yu, Clinton | ko |
dc.contributor.author | Ta, Hai | ko |
dc.contributor.author | Chelico, Linda | ko |
dc.contributor.author | Huang, Lan | ko |
dc.contributor.author | Gross, John | ko |
dc.contributor.author | Sali, Andrej | ko |
dc.contributor.author | Krogan, Nevan J. | ko |
dc.date.accessioned | 2021-09-28T02:11:02Z | - |
dc.date.available | 2021-09-28T02:11:02Z | - |
dc.date.created | 2021-09-28 | - |
dc.date.created | 2021-09-28 | - |
dc.date.created | 2021-09-28 | - |
dc.date.issued | 2021-08 | - |
dc.identifier.citation | MOLECULAR & CELLULAR PROTEOMICS, v.20 | - |
dc.identifier.issn | 1535-9476 | - |
dc.identifier.uri | http://hdl.handle.net/10203/287913 | - |
dc.description.abstract | Structural analysis of host-pathogen protein complexes remains challenging, largely due to their structural heterogeneity. Here, we describe a pipeline for the structural characterization of these complexes using integrative structure modeling based on chemical cross-links and residue-protein contacts inferred from mutagenesis studies. We used this approach on the HIV-1 Vif protein bound to restriction factor APOBEC3G (A3G), the Cullin-5 E3 ring ligase (CRL5), and the cellular transcription factor Core Binding Factor Beta (CBF beta) to determine the structure of the (A3G-Vif-CRL5-CBF beta) complex. Using the MS-cleavable DSSO cross-linker to obtain a set of 132 cross-links within this reconstituted complex along with the atomic structures of the subunits and mutagenesis data, we computed an integrative structure model of the heptameric A3G-Vif-CRL5-CBF beta complex. The structure, which was validated using a series of tests, reveals that A3G is bound to Vif mostly through its N-terminal domain. Moreover, the model ensemble quantifies the dynamic heterogeneity of the A3G C-terminal domain and Cul5 positions. Finally, the model was used to rationalize previous structural, mutagenesis and functional data not used for modeling, including information related to the A3G-bound and unbound structures as well as mapping functional mutations to the A3G-Vif interface. The experimental and computational approach described here is generally applicable to other challenging host-pathogen protein complexes. | - |
dc.language | English | - |
dc.publisher | ELSEVIER | - |
dc.title | Characterization of an A3G-Vif(HIV-1)-CRL5-CBF beta Structure Using a Cross-linking Mass Spectrometry Pipeline for Integrative Modeling of Host-Pathogen Complexes | - |
dc.type | Article | - |
dc.identifier.wosid | 000697557100001 | - |
dc.type.rims | ART | - |
dc.citation.volume | 20 | - |
dc.citation.publicationname | MOLECULAR & CELLULAR PROTEOMICS | - |
dc.identifier.doi | 10.1016/j.mcpro.2021.100132 | - |
dc.contributor.localauthor | Kim, Seung Joong | - |
dc.contributor.nonIdAuthor | Kaake, Robyn M. | - |
dc.contributor.nonIdAuthor | Echeverria, Ignacia | - |
dc.contributor.nonIdAuthor | Von Dollen, John | - |
dc.contributor.nonIdAuthor | Chesarino, Nicholas M. | - |
dc.contributor.nonIdAuthor | Feng, Yuqing | - |
dc.contributor.nonIdAuthor | Yu, Clinton | - |
dc.contributor.nonIdAuthor | Ta, Hai | - |
dc.contributor.nonIdAuthor | Chelico, Linda | - |
dc.contributor.nonIdAuthor | Huang, Lan | - |
dc.contributor.nonIdAuthor | Gross, John | - |
dc.contributor.nonIdAuthor | Sali, Andrej | - |
dc.contributor.nonIdAuthor | Krogan, Nevan J. | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | HUMAN-IMMUNODEFICIENCY-VIRUS | - |
dc.subject.keywordPlus | SINGLE AMINO-ACID | - |
dc.subject.keywordPlus | APOBEC3G CATALYTIC DOMAIN | - |
dc.subject.keywordPlus | UBIQUITIN LIGASE COMPLEX | - |
dc.subject.keywordPlus | VIRAL INFECTIVITY FACTOR | - |
dc.subject.keywordPlus | HIV-1 VIF | - |
dc.subject.keywordPlus | TYPE-1 VIF | - |
dc.subject.keywordPlus | CBF-BETA | - |
dc.subject.keywordPlus | MOLECULAR ARCHITECTURE | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
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