DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Sukmo | ko |
dc.contributor.author | Kim, Yujin | ko |
dc.contributor.author | Shin, Yumi | ko |
dc.contributor.author | Song, Ji-Joon | ko |
dc.contributor.author | Jon, Sangyong | ko |
dc.date.accessioned | 2021-07-19T07:10:23Z | - |
dc.date.available | 2021-07-19T07:10:23Z | - |
dc.date.created | 2021-07-19 | - |
dc.date.created | 2021-07-19 | - |
dc.date.issued | 2021-06 | - |
dc.identifier.citation | ACS NANO, v.15, no.6, pp.10722 - 10732 | - |
dc.identifier.issn | 1936-0851 | - |
dc.identifier.uri | http://hdl.handle.net/10203/286735 | - |
dc.description.abstract | Although naturally occurring, self-assembled protein nanoarchitectures have been utilized as antigen-delivery carriers, and the inability of such carriers to elicit immunogenicity requires additional use of strong adjuvants. Here, we report an immunogenic Brucella outer membrane protein BP26-derived nanoarchitecture displaying the influenza extracellular domain of matrix protein-2 (M2e) as a vaccine platform against influenza virus. Genetic engineering of a monomeric BP26 containing four or eight tandem repeats of M2e resulted in a hollow barrel-shaped nanoarchitecture (BP26-M2e nanobarrel). Immunization with BP26-M2e nanobarrels induced a strong M2e-specific humoral immune response in vivo that was much greater than that of a physical mixture of soluble M2e and BP26, with or without the use of an alum adjuvant. An anti-M2e antibody generated by BP26-M2e nanobarrel-immunized mice specifically bound to influenza virus-infected cells. Furthermore, in viral challenge tests, BP26-M2e nanobarrels effectively protected mice from influenza virus infection-associated death, even without the use of a conventional adjuvant. A mechanism study revealed that both M2e-specific antibody-dependent cellular cytotoxicity and T cell responses are involved in the vaccine efficacy of BP26-M2e nanobarrels. These findings suggest that the BP26-based nanobarrel developed here represents a versatile vaccine platform that can be used against various viral infections. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Antigen-Presenting, Self-Assembled Protein Nanobarrels as an Adjuvant-Free Vaccine Platform against Influenza Virus | - |
dc.type | Article | - |
dc.identifier.wosid | 000665748900136 | - |
dc.identifier.scopusid | 2-s2.0-85108650236 | - |
dc.type.rims | ART | - |
dc.citation.volume | 15 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 10722 | - |
dc.citation.endingpage | 10732 | - |
dc.citation.publicationname | ACS NANO | - |
dc.identifier.doi | 10.1021/acsnano.1c04078 | - |
dc.contributor.localauthor | Song, Ji-Joon | - |
dc.contributor.localauthor | Jon, Sangyong | - |
dc.contributor.nonIdAuthor | Shin, Yumi | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | brucella BP26 | - |
dc.subject.keywordAuthor | antiviral vaccine | - |
dc.subject.keywordAuthor | nanovaccine | - |
dc.subject.keywordAuthor | influenza virus | - |
dc.subject.keywordAuthor | humoral immunity | - |
dc.subject.keywordPlus | BRUCELLA-MELITENSIS | - |
dc.subject.keywordPlus | PROTECTION | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | BP26 | - |
dc.subject.keywordPlus | CYTOTOXICITY | - |
dc.subject.keywordPlus | EPITOPES | - |
dc.subject.keywordPlus | FEATURES | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordPlus | M2 | - |
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