DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Byoung-Hoon | ko |
dc.contributor.author | Lee, Youngseop | ko |
dc.contributor.author | Yu, Eun-Sil | ko |
dc.contributor.author | Na, Hamin | ko |
dc.contributor.author | Kang, Minhee | ko |
dc.contributor.author | Huh, Hee Jae | ko |
dc.contributor.author | Jeong, Ki-Hun | ko |
dc.date.accessioned | 2021-07-19T06:10:09Z | - |
dc.date.available | 2021-07-19T06:10:09Z | - |
dc.date.created | 2021-07-19 | - |
dc.date.created | 2021-07-19 | - |
dc.date.created | 2021-07-19 | - |
dc.date.issued | 2021-06 | - |
dc.identifier.citation | ACS NANO, v.15, no.6, pp.10194 - 10202 | - |
dc.identifier.issn | 1936-0851 | - |
dc.identifier.uri | http://hdl.handle.net/10203/286727 | - |
dc.description.abstract | Advent and fast spread of pandemic diseases draw worldwide attention to rapid, prompt, and accurate molecular diagnostics with technical development of ultrafast polymerase chain reaction (PCR). Microfluidic on-chip PCR platforms provide highly efficient and small-volume bioassay for point-of-care diagnostic applications. Here we report ultrafast, real-time, and on-chip nanoplasmonic PCR for rapid and quantitative molecular diagnostics at point-of-care level. The plasmofluidic PCR chip comprises glass nanopillar arrays with Au nanoislands and gas-permeable microfluidic channels, which contain reaction microchamber arrays, a precharged vacuum cell, and a vapor barrier. The on-chip configuration allows both spontaneous sample loading and microbubble-free PCR reaction during which the plasmonic nanopillar arrays result in ultrafast photothermal cycling. After rapid sample loading less than 3 min, two-step PCR results for 40 cycles show rapid amplification in 264 s for lambda-DNA, and 306 s for plasmids expressing SARS-CoV-2 envelope protein. In addition, the in situ cyclic real-time quantification of amplicons clearly demonstrates the amplification efficiencies of more than 91%. This PCR platform can provide rapid point-of-care molecular diagnostics in helping slow the fast-spreading pandemic. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Ultrafast and Real-Time Nanoplasmonic On- Chip Polymerase Chain Reaction for Rapid and Quantitative Molecular Diagnostics | - |
dc.type | Article | - |
dc.identifier.wosid | 000665748900086 | - |
dc.identifier.scopusid | 2-s2.0-85108296063 | - |
dc.type.rims | ART | - |
dc.citation.volume | 15 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 10194 | - |
dc.citation.endingpage | 10202 | - |
dc.citation.publicationname | ACS NANO | - |
dc.identifier.doi | 10.1021/acsnano.1c02154 | - |
dc.contributor.localauthor | Jeong, Ki-Hun | - |
dc.contributor.nonIdAuthor | Kang, Minhee | - |
dc.contributor.nonIdAuthor | Huh, Hee Jae | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | photothermal effect | - |
dc.subject.keywordAuthor | nanoplasmonic | - |
dc.subject.keywordAuthor | rapid PCR | - |
dc.subject.keywordAuthor | on-chip PCR | - |
dc.subject.keywordAuthor | SARS-CoV-2 | - |
dc.subject.keywordPlus | PCR SYSTEM | - |
dc.subject.keywordPlus | POINT | - |
dc.subject.keywordPlus | VALIDATION | - |
dc.subject.keywordPlus | POWER | - |
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