DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yeo, In-Seok | ko |
dc.contributor.author | Cho, Byung-Kwan | ko |
dc.contributor.author | Kim, Jung Hoe | ko |
dc.date.accessioned | 2021-06-03T05:10:21Z | - |
dc.date.available | 2021-06-03T05:10:21Z | - |
dc.date.created | 2021-03-11 | - |
dc.date.created | 2021-03-11 | - |
dc.date.issued | 2021-06 | - |
dc.identifier.citation | BIOPROCESS AND BIOSYSTEMS ENGINEERING, v.44, no.6, pp.1147 - 1154 | - |
dc.identifier.issn | 1615-7591 | - |
dc.identifier.uri | http://hdl.handle.net/10203/285504 | - |
dc.description.abstract | l-Ribose, a starting material for the synthesis of l-nucleoside, has attracted lots of attention since l-nucleoside is responsible for the antiviral activities of the racemic mixtures of nucleoside enantiomers. In this study, the l-ribulose-producing Candida tropicalis strain was engineered for the conversion of l-arabinose to l-ribose. For the construction of a uracil auxotroph, the URA3 gene was excised by homologous recombination. The expression cassette of codon-optimized l-ribose isomerase gene from Acinetobacter calcoaceticus DL-28 under the control of the GAPDH promoter was integrated to the uracil auxotroph. The resulting strain, K1 CoSTP2 LsaAraA AcLRI, was cultivated with the glucose/l-arabinose mixture. At 45.5 h of fermentation, 6.0 g/L of l-ribose and 3.2 g/L of l-ribulose were produced from 30 g/L of l-arabinose. The proportion between l-ribose and l-ribulose was approximately 2:1 and the conversion yield of l-arabinose to l-ribose was about 20% (w/w). The l-ribose-producing yeast strain was successfully constructed for the first time and could convert l-arabinose to l-ribose in one-pot fermentation using the mixture of glucose and l-arabinose. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.title | Conversion of l-arabinose to l-ribose by genetically engineered Candida tropicalis | - |
dc.type | Article | - |
dc.identifier.wosid | 000616472500001 | - |
dc.identifier.scopusid | 2-s2.0-85100780750 | - |
dc.type.rims | ART | - |
dc.citation.volume | 44 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 1147 | - |
dc.citation.endingpage | 1154 | - |
dc.citation.publicationname | BIOPROCESS AND BIOSYSTEMS ENGINEERING | - |
dc.identifier.doi | 10.1007/s00449-020-02506-2 | - |
dc.contributor.localauthor | Cho, Byung-Kwan | - |
dc.contributor.localauthor | Kim, Jung Hoe | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Candida tropicalis | - |
dc.subject.keywordAuthor | l-Arabinose | - |
dc.subject.keywordAuthor | l-Ribose | - |
dc.subject.keywordAuthor | l-Ribose isomerase | - |
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