DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jiang, Xinglin | ko |
dc.contributor.author | Radko, Yulia | ko |
dc.contributor.author | Gren, Tetiana | ko |
dc.contributor.author | Palazzotto, Emilia | ko |
dc.contributor.author | Jorgensen, Tue Sparholt | ko |
dc.contributor.author | Cheng, Tao | ko |
dc.contributor.author | Xian, Mo | ko |
dc.contributor.author | Weber, Tilmann | ko |
dc.contributor.author | Lee, Sang Yup | ko |
dc.date.accessioned | 2021-05-25T05:50:05Z | - |
dc.date.available | 2021-05-25T05:50:05Z | - |
dc.date.created | 2021-05-25 | - |
dc.date.created | 2021-05-25 | - |
dc.date.created | 2021-05-25 | - |
dc.date.issued | 2021-05 | - |
dc.identifier.citation | APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.87, no.10, pp.1 - 8 | - |
dc.identifier.issn | 0099-2240 | - |
dc.identifier.uri | http://hdl.handle.net/10203/285319 | - |
dc.description.abstract | epsilon-Poly-L-lysine is a potent antimicrobial produced through fermentation of Streptomyces and used in many Asian countries as a food preservative. It is synthesized and excreted by a special nonribosomal peptide synthetase (NRPS)-like enzyme called Pls. In this study, we discovered a gene from cheese bacterium Corynebacterium variabile that showed high similarity to the Pls from Streptomyces in terms of domain architecture and gene context. By cloning it into Streptomyces coelicolor with a Streptomyces albulus Pls promoter, we confirmed that its product is indeed epsilon-poly-L-lysine. A comprehensive sequence analysis suggested that Pls genes are widely spread among coryneform actinobacteria isolated from cheese and human skin; 14 out of 15 Brevibacterium isolates and 10 out of 12 Corynebacterium isolates contain it in their genomes. This finding raises the possibility that epsilon-poly-L-lysine as a bioactive secondary metabolite might be produced and play a role in the cheese and skin ecosystems. IMPORTANCE Every year, microbial contamination causes billions of tons of food wasted and millions of cases of illness. epsilon-Poly-L-lysine has potent, wide-spectrum inhibitory activity and is heat stable and biodegradable. It has been approved for food preservation by an increasing number of countries. epsilon-Poly-L-lysine is produced from soil bacteria of the genus Streptomyces, also producers of various antibiotic drugs and toxins and not considered to be a naturally occurring food component. The frequent finding of pls in cheese and skin bacteria suggests that epsilon-poly-L-lysine may naturally exist in cheese and on our skin, and epsilon-poly-L-lysine producers are not limited to filamentous actinobacteria. | - |
dc.language | English | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.title | Distribution of ε-Poly-l-Lysine Synthetases in Coryneform Bacteria Isolated from Cheese and Human Skin | - |
dc.type | Article | - |
dc.identifier.wosid | 000645866700011 | - |
dc.identifier.scopusid | 2-s2.0-85105833605 | - |
dc.type.rims | ART | - |
dc.citation.volume | 87 | - |
dc.citation.issue | 10 | - |
dc.citation.beginningpage | 1 | - |
dc.citation.endingpage | 8 | - |
dc.citation.publicationname | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | - |
dc.identifier.doi | 10.1128/AEM.01841-20 | - |
dc.contributor.localauthor | Lee, Sang Yup | - |
dc.contributor.nonIdAuthor | Jiang, Xinglin | - |
dc.contributor.nonIdAuthor | Radko, Yulia | - |
dc.contributor.nonIdAuthor | Gren, Tetiana | - |
dc.contributor.nonIdAuthor | Palazzotto, Emilia | - |
dc.contributor.nonIdAuthor | Jorgensen, Tue Sparholt | - |
dc.contributor.nonIdAuthor | Cheng, Tao | - |
dc.contributor.nonIdAuthor | Xian, Mo | - |
dc.contributor.nonIdAuthor | Weber, Tilmann | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | epsilon-poly-L-lysine | - |
dc.subject.keywordAuthor | NRPS | - |
dc.subject.keywordAuthor | cheese bacteria | - |
dc.subject.keywordAuthor | skin bacteria | - |
dc.subject.keywordAuthor | antimicrobial | - |
dc.subject.keywordAuthor | food preservative | - |
dc.subject.keywordAuthor | epsilon-poly-L-lysine | - |
dc.subject.keywordAuthor | epsilon-polylysine | - |
dc.subject.keywordAuthor | polylysine | - |
dc.subject.keywordPlus | STREPTOMYCES | - |
dc.subject.keywordPlus | POLY(EPSILON-L-LYSINE) | - |
dc.subject.keywordPlus | POLYLYSINE | - |
dc.subject.keywordPlus | ACID) | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | STRAINS | - |
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