A study on the demonstration and application of the on-chip optical biosensor based on active microdisk resonators활성 미세 원반 공진기를 기반으로 한 온-칩 광 바이오 센서의 제작과 응용에 관한 연구

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Label-free biosensors based on whispering gallery mode (WGM) resonators have been continuously studied over the past decade in virtue of their high sensitivity and high-quality factor. However, there is still a considerable gap between its academic achievements and practical applications, which require not only high sensitivity but also low cost and ease of use. In this research, WGM resonator sensors integrated with microfluidic circuits are demonstrated in simple, practical form released from strict coupling scheme of tapered fibers, which have long been considered as an essential element but the main hurdle for real applications. By taking advantage of large absorption cross-section of silicon nanocluster, pump light exposed from the top of silicon-rich silicon nitride (SRSN) resonators can efficiently induce light emission spectrum peaks along with the resonant modes which are detected by a spectrometer through free-space optics. In addition, for the first time, we succeeded in acquiring the signal by exposing the LED pump light to the top of the resonator instead of the conventional laser, which significantly reduced the complexity and cost of the measurement setup. The shift of emission peaks revealing biomolecules into the measurable domain is around 6.5 times enhanced by a nano-gap structure, which strongly increases light-matter interaction by tightly confining the mode field in it. The real-time detection of the streptavidin-biotin complex experiment using a developed sensor has shown 0.012 nm/nM sensitivity, improved over 20 times larger than the previously reported WGM sensors with remote readout. The real-time sensing experiments for nucleic acid of new infectious pathogens, such as ZIKV virus, and the bacterial species Brucella ovis have shown that a single copy of the nucleic acid can be detected within 10 minutes. Finally, we propose an alternative detection method for determining the concentration of a biomolecule based on the Langmuir adsorption model. We demonstrate that the proposed method determines the concentration relative to a reference sample with the required accuracy, with a single measurement at any point in time.
Advisors
Lee, Hansuekresearcher이한석researcher
Description
한국과학기술원 :물리학과,
Publisher
한국과학기술원
Issue Date
2020
Identifier
325007
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 물리학과, 2020.8,[vii, 66 p. :]

Keywords

Optical biosensor▼aDisk resonator▼aNano-gap structure▼aLangmuir adsorption model▼aBalanced detection method; 광 바이오 센서▼a디스크 공진기▼a나노-갭▼a랑뮈에 흡착 모델▼a균형감지법

URI
http://hdl.handle.net/10203/284331
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=924321&flag=dissertation
Appears in Collection
PH-Theses_Ph.D.(박사논문)
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