Development of optogenetic system for regulating transmembrane receptors and application광유전학을 통한 세포 막 수용체의 활성 조절 및 연구

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dc.contributor.advisorHeo, Won Do-
dc.contributor.advisor허원도-
dc.contributor.authorLee, Minji-
dc.date.accessioned2021-05-12T19:42:26Z-
dc.date.available2021-05-12T19:42:26Z-
dc.date.issued2018-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=919515&flag=dissertationen_US
dc.identifier.urihttp://hdl.handle.net/10203/284280-
dc.description학위논문(박사) - 한국과학기술원 : 생명과학과, 2018.8,[iv, 77 p. :]-
dc.description.abstractCells sense and transfer extracellular information to intracellular biochemical context through transmembrane receptors. External signaling molecules interact with cell surface receptors and regulate diverse intracellular processes. Because multiple-steps characteristic and complexity of signaling molecules, receptor mediated cellular functions were difficult to study in high spatiotemporal manner. In chapter 1, I established the optogenetic toolkits for regulating the activity of FGF receptor family (FGFR1-FGFR4) using Arabidopsis thaliana’s cryptochrome 2 homo-interaction. Extending concept of OptoFGFR1 to other members of FGFR family, I generated light-inducible FGF receptors, OptoFGFRs (OptoFGFR1-4). Using this fine-tuned module systems, I investigated the different role of FGFR signaling in endothelial cell migration and glia function, especially astrocyte activity. For further in vivo application, adeno-associated virus (AAV) was used for expression of optically-controlled receptor in the central nervous system. In chapter 2, I introduce OptoTGFBRs, a new optogenetic system to regulate Transforming growth factor β Receptor signaling pathway with spatiotemporal manner. Using blue light dependent hetero-interaction between Arabidopsis thaliana’s cryptochrome 2 and CIB1 protein, I develop chimeric TGF- $\beta$ receptor. Upon light illumination, OptoTGFBRs efficiently activates downstream signaling pathways, Smad2 phosphorylation and related gene expression.-
dc.languageeng-
dc.publisher한국과학기술원-
dc.subjectOptogenetics▼aFibroblast Growth Factor Receptor▼aTransforming growth factor $\beta$ Receptor▼aOptoFGFRs▼aOptoTGFBRs-
dc.subject광유전학▼a섬유아세포성장인자 수용체▼a형질전환생장인자 수용체▼a광유도 섬유아세포성장인자 수용체▼a광유도 형질전환인자 수용체-
dc.titleDevelopment of optogenetic system for regulating transmembrane receptors and application-
dc.title.alternative광유전학을 통한 세포 막 수용체의 활성 조절 및 연구-
dc.typeThesis(Ph.D)-
dc.identifier.CNRN325007-
dc.description.department한국과학기술원 :생명과학과,-
dc.contributor.alternativeauthor이민지-
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