Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins항체광유전학 기술 개발을 통한 세포 내 단백질 기능 연구

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dc.contributor.advisorHeo, Won Do-
dc.contributor.advisor허원도-
dc.contributor.authorYu, Daseuli-
dc.date.accessioned2021-05-12T19:40:27Z-
dc.date.available2021-05-12T19:40:27Z-
dc.date.issued2020-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=909397&flag=dissertationen_US
dc.identifier.urihttp://hdl.handle.net/10203/284170-
dc.description학위논문(박사) - 한국과학기술원 : 생명과학과, 2020.2,[v, 99 p. :]-
dc.description.abstractIntracellular antibodies, such as nanobodies and single-chain variable fragments (scFv), have become powerful tools for imaging, modulating, and neutralizing endogenous target proteins. Although a variety of antibody engineering techniques have been developed, an optogenetic tool for activating intracellular antibody to precisely control its target protein has not been reported. In chapter 1, I describe an optogenetically activatable intracellular antibody (Optobody) consisting of split antibody fragments and blue light-mediated heterodimerization domains. Blue light stimulation activates the optobody, inducing it to capture its target protein and subsequently inhibit the target. In chapter 2, I expanded this optobody platform by generating various optobodies from previously developed intracellular antibodies. In chapter 3, I demonstrated that photoactivation of a gelsolin (GSN) optobody and $\beta$2 adrenergic receptor ($\beta$2AR) optobody shut down endogenous GSN activity and $\beta$2AR signaling, respectively. Applying the novel optogenetic platform to the broad pool of available intracellular antibodies will facilitate optogenetic manipulation of various endogenous proteins and may provide a basis for designing potential inducible drugs.-
dc.languageeng-
dc.publisher한국과학기술원-
dc.subjectOptogenetics▼aAntibody fragments▼aNanobody▼ascFv▼aOptobody▼aEndogenous protein inhibition▼aAntibody-based drug-
dc.subject광유전학▼a항체 조각▼a나노바디▼ascFv▼a광활성 항체▼a옵토바디▼a내생단백질 기능 저해▼a항체 의약품-
dc.titleOptogenetic activation of intracellular antibodies for direct modulation of endogenous proteins-
dc.title.alternative항체광유전학 기술 개발을 통한 세포 내 단백질 기능 연구-
dc.typeThesis(Ph.D)-
dc.identifier.CNRN325007-
dc.description.department한국과학기술원 :생명과학과,-
dc.contributor.alternativeauthor유다슬이-
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