DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kang, Chang-Won | - |
dc.contributor.advisor | 강창원 | - |
dc.contributor.author | Lee, Bum-Yong | - |
dc.contributor.author | 이범용 | - |
dc.date.accessioned | 2011-12-12T08:59:27Z | - |
dc.date.available | 2011-12-12T08:59:27Z | - |
dc.date.issued | 1993 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=68313&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28401 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생명과학과, 1993.2, [ vi, 47 p. ] | - |
dc.description.abstract | Ribozymes are potentially very powerful agents for perturbing intracellular gene expression. Hammerhead structure ribozymes were designed to cleave the mRNA of hepatitis B virus X gene. Its cleavage was designed to result in the suppressed expression of lacZ fused to a 5$^\prime$462-bp fragment of X gene. The hammerhead ribozymes consist of a core region for cleavage activity and two variable regions for binding to a target RNA sequenec. A target region of X mRNA was chosen among 12 GUC-containing regions, because it is predicted not to be base-paired by a computer algorthm of RNA secondary structure prediction. Each target-binding, variable region was designed to contain 4 matched bases near the core sequence and 4 2-base wobbles (matched and mismatched) in the rest. Among 256 possible sequences 12 sequences have so far been identified to yield suppressed expression of the fused lacZ. The $\beta$-galactosidase activities were reduced at least 10-fold. Also corresponding polypeptedes were not produced as revealed by SDS-polyacrylamide gel electrophoresis. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | 촉매활성 RNA. | - |
dc.title | Synthetic ribozyme suppressing HBV X gene expression | - |
dc.title.alternative | 합성된 리보핵산효소에 의한 HBV X 유전자 발현 억제 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 68313/325007 | - |
dc.description.department | 한국과학기술원 : 생명과학과, | - |
dc.identifier.uid | 000911420 | - |
dc.contributor.localauthor | Kang, Chang-Won | - |
dc.contributor.localauthor | 강창원 | - |
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