DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kim, Hyoung-Man | - |
dc.contributor.advisor | 김형만 | - |
dc.contributor.author | Song, Maeng-Seok | - |
dc.contributor.author | 송맹석 | - |
dc.date.accessioned | 2011-12-12T08:58:56Z | - |
dc.date.available | 2011-12-12T08:58:56Z | - |
dc.date.issued | 1991 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=67702&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28365 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물공학과, 1991.2, [ v, 33 p. ] | - |
dc.description.abstract | In contrast to easlier reports of complete translocation, apocytochrome c is found to be only partially translocated across the bilayer of PS vesicles entrapped with trypsin. A 2.5 kDa polypeptide segment of C-terminal area of apocytochrome c (80-104) remains attasched to the bilayer after the rest of the apocytochrome c is digested by internal trypsin. Digestion of vesicle-bound apocytochrome c by externally added trypsin also yielded a protected segment in the C-terminal area (74-104). These observations suggest that apocytochrome c spans the bilayer when it binds to PS vesicles and there is a partial translocation when it is being digested by either external or internal trypsin. Externally added apocytochrome c was hydrophobically labeled with [I] TID and treated with endoproteinase Arg C. The results indicates that the N-terminal area (1-38) penetrates the bilayer. The vesicle-bound apocytochrome c was also digested with aminopeptidase showing that the N-terminal end is exposed to the outside. A dynamic interaction between apocytochrome c and vesicles and a possible partial translocation model are discussed. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | Partial translocation of apocytochrome c across phospholipid bilayer caused by tryptic digestion | - |
dc.title.alternative | Trypsin에 의한 apocytochrome c 의 인지질 막에 대한 부분적 전위에 관한 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 67702/325007 | - |
dc.description.department | 한국과학기술원 : 생물공학과, | - |
dc.identifier.uid | 000891237 | - |
dc.contributor.localauthor | Kim, Hyoung-Man | - |
dc.contributor.localauthor | 김형만 | - |
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