Development of an siRNA delivery system using a cancer-specific cell-penetrating peptide암세포 특이적 세포투과 펩타이드를 이용한 siRNA 전달체 개발 연구

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RNA interference provides an effective tool for developing antitumor therapies. Cell-penetrating peptides (CPPs) are delivery vectors widely used to efficiently transport small-interfering RNA (siRNA) to intracellular targets. In this study, we investigated the efficacy of the cancer-specific CPP carrier BR2 to specifically transport siRNA to cancer-target cells. Our results showed that BR2 formed a complex with anti-vascular endothelial growth factor siRNA (siVEGF) that exhibited the appropriate size and surface charge for in vivo treatment. Additionally, the BR2-VEGF siRNA complex exhibited significant serum stabil-ity and high levels of gene-silencing effects in vitro. Moreover, the transfection efficiency of the complex into a cancer cell line was higher than that observed in non-cancer cell lines, resulting in downregulated in-tracellular VEGF levels in HeLa cells and comprehensively improved antitumor efficacy in the absence of significant toxicity. These results indicated that BR2 has significant potential for safe, efficient, and specific delivery of siRNA for diverse applications.
Advisors
Kim, Sun Changresearcher김선창researcher
Description
한국과학기술원 :생명과학과,
Publisher
한국과학기술원
Issue Date
2018
Identifier
325007
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명과학과, 2018.2,[vi, 112 p. :]

Keywords

RNA interference▼asmall-interfering RNA▼acell-penetrating peptide▼acancer specific peptide▼aVEGF; 예쁜꼬마선충▼a이중가닥RNA▼asiRNA▼aRNA 간섭현상▼a세포투과 펩타이드

URI
http://hdl.handle.net/10203/283408
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=886200&flag=dissertation
Appears in Collection
BS-Theses_Ph.D.(박사논문)
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