DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kang, Chang-Won | - |
dc.contributor.advisor | 강창원 | - |
dc.contributor.author | Kang, Sin-Ae | - |
dc.contributor.author | 강신애 | - |
dc.date.accessioned | 2011-12-12T08:58:28Z | - |
dc.date.available | 2011-12-12T08:58:28Z | - |
dc.date.issued | 1990 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=67170&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28333 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물공학과, 1990.2, [ v, 60 p. ] | - |
dc.description.abstract | The nucleotide sequence of the full length canine gastrin gene and its flanking regions were determined. The 2.3-kb gene consists of three exons and two introns. The 300 bp upstream flanking region sequence reveals some transcription regulatory sequences. Also the 450 bp downstream sequence reveals a putative transcription terminator sequence. The 1.7 kb intron I, about half the size of human intron I, is located in the 5`` untranslated region and the 117 bp intron II interrupts the aspartic acid codon at position 71 of preprogastrin. All the intron-exon junctions contain the consensus sequences of the 5`` and 3`` ends of eucaryotic introns, 5`` GT and AG 3`` respectively. A putative transcription initiation site is assigned to the adenine at 64 bp upstream from the first exon-inron junction on the basis of sequence comparison of human and canine gastrin promoters. A possible ``TATA`` equivalent sequence TTATAA is located at 28 bp upstream from the putative cap site. However, ``CAAT`` box equivalent sequence is not found in either orientation. A ploy(A) addition signal, AAUAAA, is located at 62 base pairs downstream from the termination codon UAG. The 5`` regulatory sequence also contains Ap-1 and Ap-2 binding sites, which suggests transcription regulation by these transcription factors. A putative terminator, TTTTTAATATTTTACTTATTTATTTATTT, which resembles the human terminator sequence TTTTTTTTTAATTTTTATTTTATTTTATTTT is located at approximately 160 bp downstream of the ploy(A) addition site. It has a 6.5 bp inverted repeat sequence unlike the 10.5 bp inverted repeat symmetry of human gastrin terminator. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | Structure and nucleotide sequence of canine gastrin gene | - |
dc.title.alternative | 개 가스트린 유전자의 구조 및 염기서열 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 67170/325007 | - |
dc.description.department | 한국과학기술원 : 생물공학과, | - |
dc.identifier.uid | 000881008 | - |
dc.contributor.localauthor | Kang, Chang-Won | - |
dc.contributor.localauthor | 강창원 | - |
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