DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Lee, Daeyoup | - |
dc.contributor.advisor | 이대엽 | - |
dc.contributor.author | Han, Sungwook | - |
dc.date.accessioned | 2021-05-11T19:37:17Z | - |
dc.date.available | 2021-05-11T19:37:17Z | - |
dc.date.issued | 2019 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=871380&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/283219 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생명과학과, 2019.8,[v, 120 p. :] | - |
dc.description.abstract | CTCF plays a critical role in maintaining the three-dimensional (3D) chromatin organization, which is important for gene regulation, as it allows distal regulatory elements to come into proximity with one another. However, the detailed mechanism responsible for establishing and maintaining the recruitment of CTCF remains elusive. Here, we use in situ Hi-C to show that the ATP-dependent chromatin remodeler, Chd4, regulates intra-chromatin looping by controlling chromatin accessibility to conceal aberrant CTCF-binding sites in mouse embryonic stem cells (mESCs). These aberrant CTCF-binding sites are embedded in B2 SINEs and are localized within the interior of chromatin loops. In the absence of Chd4, the aberrant CTCF-binding sites become accessible and improper CTCF recruitment occurs, resulting in disorganization of the 3D chromatin architecture and subsequent disruption of enhancer-promoter interactions and the transcription of the corresponding genes. These results indicate that Chd4 regulates adequate transcription of mESCs by securing the proper 3D chromatin organization. These results highlight the Chd4-mediated maintenance of 3D chromatin organization is required for adequate transcription in mESCs. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | 쥐 배아 줄기 세포▼a3차원 염색질 구조▼a염색질 내 루프 구조▼ain situ Hi-C▼aCTCF▼aChd4▼aATP-의존성 염색질 리모델러▼a염색질 접근성▼aB2 SINE▼a인핸서-프로모터 상호작용 | - |
dc.subject | mouse embryonic stem cell▼athree dimensional chromatin architecture▼aintra chromatin loop▼ain situ Hi-C▼aCTCF▼aChd4▼aATP-dependent chromatin remodelers▼achromatin accessibility▼aB2 SINE▼aEnhancer-promoter interactions | - |
dc.title | Mechanistic study of mammalian CHD4 in 3D chromatin structure and topology | - |
dc.title.alternative | 포유류 CHD4 단백질이 관여하는 크로마틴 구조와 3차원 위상에 관한 연구 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :생명과학과, | - |
dc.contributor.alternativeauthor | 한성욱 | - |
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