DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Byun, Si-Myung | - |
dc.contributor.advisor | 변시명 | - |
dc.contributor.author | Kang, Dae-Ook | - |
dc.contributor.author | 강대욱 | - |
dc.date.accessioned | 2011-12-12T08:58:03Z | - |
dc.date.available | 2011-12-12T08:58:03Z | - |
dc.date.issued | 1989 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=66642&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28305 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물공학과, 1989.2, [ vii, 60 p. ] | - |
dc.description.abstract | To clone a thermostable subtilisin-like protease gene, Chromosomal DNA was isolated from various thermophilic Bacillus species such as Bacillus coagulans NRRL B-5666, Bacillus licheniformis ATCC 27811, Bacillus stearothermophilus ATCC 12980 and Bacillvs Subtilis JCM 2889. The chromosomal DNA was digested with EcoR I and hybridized using aprA gene from Bacillus subtilis as a probe. The strong signal sized about 6.7kb appeared in B. coagulans. B. coagulans chromosomal DNA digested with EcoR I was electroeluted into 7 fractions and hybridized. F. II showed the positive signal and ligated with pUC9 and transformed into E. coli JM83. About 8000 transformants were screened to obtain recombianat with subtilisin-like protease gene by colony hybridization. Positive signal emerged in three transformants and these are rescreened by Southern hybridization. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | Cloning of thermostable subtilisin-like protease gene from thermophilic bacillus species | - |
dc.title.alternative | 호열성 Bacillus 균주로부터 열안정성 유사 subtilisin 유전자의 클로닝 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 66642/325007 | - |
dc.description.department | 한국과학기술원 : 생물공학과, | - |
dc.identifier.uid | 000871003 | - |
dc.contributor.localauthor | Byun, Si-Myung | - |
dc.contributor.localauthor | 변시명 | - |
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