DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kang, Chang-Won | - |
dc.contributor.advisor | 강창원 | - |
dc.contributor.author | Lee, In-Woo | - |
dc.contributor.author | 이인우 | - |
dc.date.accessioned | 2011-12-12T08:57:49Z | - |
dc.date.available | 2011-12-12T08:57:49Z | - |
dc.date.issued | 1988 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=66131&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28289 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물공학과, 1988.2, [ v, 40 p. ] | - |
dc.description.abstract | The in vitro transcripts of phage SP6 RNA polymerase usually contain extraneous 5`` and 3`` plasmid sequences in addition to the cloned gene sequence. Template plasmids were constructed to eliminate these extraneous sequences. The 5 S RNA gene of Xenopus borealis somatic cell was inserted at the transcription initiation site (BamHI site) of PCKSP6 so that the transcription starts from the 5`` end of the gene. The restriction enzyme DraI site was inserted at the 3`` end of 5 S RNA structrual gene by oligonucleotide-directed site-specific mutagenesis. Now, when the recombinant DNA is digested with DraI, the resulting in vitro run-off transcripts of the linearized template using phage SP6 RNA polymerase contain the authentic 5`` and 3``ends. Thus, the large quantity of authentic 5 S RNA and any mutant RNAs, which could easily be radioactively labeled, can be produced for structure-function relationship studies, RNA folding studies, and other biophysical studies that have been limited by sample quantities. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | In vitro synthesis of 5 S rRNA using phage SP6 RNA polymerase | - |
dc.title.alternative | 파아지 SP6 RNA 중합효소를 이용한 5 S rRNA 의 합성 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 66131/325007 | - |
dc.description.department | 한국과학기술원 : 생물공학과, | - |
dc.identifier.uid | 000861326 | - |
dc.contributor.localauthor | Kang, Chang-Won | - |
dc.contributor.localauthor | 강창원 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.