DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Lee, Hyun-Jae | - |
dc.contributor.advisor | Yang, Kyu-Hwan | - |
dc.contributor.advisor | 이현재 | - |
dc.contributor.advisor | 양규환 | - |
dc.contributor.author | Kim, Tae-Sung | - |
dc.contributor.author | 김태성 | - |
dc.date.accessioned | 2011-12-12T08:56:53Z | - |
dc.date.available | 2011-12-12T08:56:53Z | - |
dc.date.issued | 1986 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=65034&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28225 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물공학과, 1986.2, [ viii, 54 p. ] | - |
dc.description.abstract | In this communication, we introduced a soybean trypsin inhibitor in the reaction system to block the activities of trypsin and plasmin like proteolytic enzymes. Since STI was found to be a selective inhibitor for the other serine proteases except SK-PLG complex, in the presence of STI, we were able to quantitize the activity of the SK-PLG complex using artficial chromogenic substrate such as N$^a$-CBZ-L-lysine-p-nitrophenyl exter (CLM). Also, with the kinetic study of SK-PLG complex for synthetic esters and peptides, its substrate specificity has been investigated. This results show that the SK-PLG complex is primarily specific for peptides and esters of N,C-blocking, N-positively charged amino acids which have hydrophobic groups at its a-carboxyl position. Like other serine proteases, the SK-PLG complex was irreversibly inhibited by diisopropylfluorophosphate(DIFP) and diethylpyrocarbonate(DEPC) as well. So, it was concluded that the hydroxyl group of serine and the imidazole group of histine have been constituted a part of the active site of SK-PLG complex. This result was supported by pH-profile of SK-PLG complex using N$^a$-benzoyl-L-arginine-p-nitroanilide as a substrate. And it was observed that the hydroylsis of CLN catalyzed by SK-PLG complex proceeds with an initial burst of p-nitrophenol release, followed by a slower steady-state release of this product. This experimental observation may provides proof that a particular covalent SK-PLG complex-substrate compound is formed as an intermediate in the reaction of SK-PLG complex. This, based on these results investigated, reaction mechanism of SK-PLG complex can be proposed. Also some human plasma proteins, carbonate ion, and chloride ion, etc. affected the activity of SK-PLG complex. It was considered that these components in human blood may be partially concerned in the regulation of blood fibrinolysis related to the SK-PLG complex. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | Substrate specificity and reaction model of the active complex of human plasminogen and streptokinase | - |
dc.title.alternative | Streptokinase 와 human plasminogen 의 활성 복합체에 대한 기질 특이성과 반응 기작에 관한 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 65034/325007 | - |
dc.description.department | 한국과학기술원 : 생물공학과, | - |
dc.identifier.uid | 000841080 | - |
dc.contributor.localauthor | Lee, Hyun-Jae | - |
dc.contributor.localauthor | Yang, Kyu-Hwan | - |
dc.contributor.localauthor | 이현재 | - |
dc.contributor.localauthor | 양규환 | - |
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