DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kim, Jung-Hoe | - |
dc.contributor.advisor | 김정회 | - |
dc.contributor.author | Kim, Byoung-Kwan | - |
dc.contributor.author | 김병관 | - |
dc.date.accessioned | 2011-12-12T08:56:25Z | - |
dc.date.available | 2011-12-12T08:56:25Z | - |
dc.date.issued | 1985 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=64483&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28193 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물공학과, 1985.2, [ iv, 65 p. ] | - |
dc.description.abstract | For the analysis of genetic instability and productivity of plasmid coded product in a recombinant cell fermentation system, a continuous culture was performed using a mutant of E.coli W3110 $\triangle$trp LD trp R$^{-ts}$ tna (pCRT185) harboring trp-operon. The host cell with the trp repression system possesses the resistance against feedback regulation and the temperature induction of derepression. In a batch culture, temperature were determined to be $37\,^\circ\!C$ for the optimal cell growth with repression of trp-operon (permissive temperature) and $42\,^\circ\!C$ for the maximum expression of trp-operon accompanied by derepression (nonpermissive temperature), respectively. The activity of tryptophanase was found to be 430 units/g-cell at $42\,^\circ\!C$ which was 3 times higher than that obtained at $37\,^\circ\!C$. It was also found that addition of anthranilic acid (0.5 g/l) to the medium increased the tryptophan production 11 times comparing to without its addition. In a continuous culture, specific tryptophan productivity was increased with time elapsed at a early phase and then decreased gradually due to the loss of plasmid from the host cells and rapid increase of plasmid-free cells. It was also decreased with increase of dilution rate. At dilution rate, 0.075 hr$^{-1}$, specific tryptophan productivity was found to be about 10 mg/g-cell-hr in the derepressed state, representing about thirty times increase over that found in the repressed state. From the kinetic analysis of plasmid instability in a continuous culture, it was found that the rate constant of plasmid loss was a order of magnitude of $10^{-5}$ and the increase of plasmid-free cells during the culture was mainly caused by the difference of specific growth rate between plasmid-free cells and plasmid-harboring cells rather than by the rate of plasmid loss. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | Genetic instability and tryptophan productivity in continuous culture of recombinant escherichia coli harboring trp-operon plasmid | - |
dc.title.alternative | Trp operon plasmid 를 갖는 재조합 E. coli 의 연속 배양에서 유전적 불안정성과 tryptophan 의 생산성에 관한 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 64483/325007 | - |
dc.description.department | 한국과학기술원 : 생물공학과, | - |
dc.identifier.uid | 000831053 | - |
dc.contributor.localauthor | Kim, Jung-Hoe | - |
dc.contributor.localauthor | 김정회 | - |
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