DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Juhwan | ko |
dc.contributor.author | Han, Dong Hyun | ko |
dc.contributor.author | Hwang, Sang-Hyun | ko |
dc.contributor.author | Park, Je-Kyun | ko |
dc.date.accessioned | 2021-03-26T02:12:30Z | - |
dc.date.available | 2021-03-26T02:12:30Z | - |
dc.date.created | 2020-10-05 | - |
dc.date.issued | 2020-09 | - |
dc.identifier.citation | LAB ON A CHIP, v.20, no.18, pp.3346 - 3353 | - |
dc.identifier.issn | 1473-0197 | - |
dc.identifier.uri | http://hdl.handle.net/10203/281900 | - |
dc.description.abstract | Molecular diagnostics can provide a powerful diagnostic tool since it can detect pathogens with high sensitivity, but complicated sample preparation procedures limit its widespread use as an on-site detection tool that relies on the skilled person and external equipment. To resolve these limitations, we report a solid-phase nucleic acid purification using a finger-actuated microfluidic device, which can control a set amount of flow regardless of differences in end-users. To increase the recovery rate, a finger-actuated reciprocator was newly developed and integrated into the microfluidic device that can efficiently react with silica microbeads and reagents. After verifying the finger-actuated microfluidic reciprocator, the effect of the reciprocating flow on the recovery rate was assessed to purify the standard DNA of the hepatitis B virus (HBV). The recovery rate was increased up to similar to 50% and 955 to 955 000 IU mL(-1)of HBV standard DNA was successfully purified and detected by a real-time polymerase chain reaction. Furthermore, the proposed microfluidic device was exploited to purify the HBV DNA from the patient's blood plasma samples. | - |
dc.language | English | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.title | Reciprocating flow-assisted nucleic acid purification using a finger-actuated microfluidic device | - |
dc.type | Article | - |
dc.identifier.wosid | 000569507600002 | - |
dc.identifier.scopusid | 2-s2.0-85091127174 | - |
dc.type.rims | ART | - |
dc.citation.volume | 20 | - |
dc.citation.issue | 18 | - |
dc.citation.beginningpage | 3346 | - |
dc.citation.endingpage | 3353 | - |
dc.citation.publicationname | LAB ON A CHIP | - |
dc.identifier.doi | 10.1039/d0lc00432d | - |
dc.contributor.localauthor | Park, Je-Kyun | - |
dc.contributor.nonIdAuthor | Han, Dong Hyun | - |
dc.contributor.nonIdAuthor | Hwang, Sang-Hyun | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | MEDIATED ISOTHERMAL AMPLIFICATION | - |
dc.subject.keywordPlus | DNA EXTRACTION | - |
dc.subject.keywordPlus | CHIP | - |
dc.subject.keywordPlus | MICRODEVICE | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | PCR | - |
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