DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Yang, K. H. | - |
dc.contributor.advisor | 양규환 | - |
dc.contributor.author | Kim, Hwan-Mook | - |
dc.contributor.author | 김환묵 | - |
dc.date.accessioned | 2011-12-12T08:56:04Z | - |
dc.date.available | 2011-12-12T08:56:04Z | - |
dc.date.issued | 1984 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=64049&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28169 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물공학과, 1984.2, [ [iii], 55 p. ] | - |
dc.description.abstract | Effects of T-2 toxin were studied in primary cultures of adult rat hepatocytes. Hepatocytes were isolated by a collagenase perfusion technique and cultured as monolayer in serum-free media for 48 hours. Uptake of cardiac glycoside (ouabain), hormonal induction of $\alpha$-aminoisobutyric acid (AIB) uptake and tyrosine aminotransferase (TAT) activity were used as the parameters to determine cytotoxicity. No significant lethality was observed after 6 hours treatment of 100 ng T-2 toxin/ml. At does from 10 to 100 ng/ml, T-2 toxin inhibited the induction of AIB transport and TAT activity when T-2 toxin was added simultaneously with hormones. It also exerted inhibitory effects when added at 6 hours prior to the hormonal treatment, and removed from medium. Meanwhile, T-2 toxin did not show any inhibitory effects on ouabain transport system. The results of this study suggest that T-2 toxin caused abnormality to liver functions within the testing period, but it does not due to membrane damage and lethal effect. It seems likely that T-2 toxin is a strong inhibitor of protein synthesis in rat hepatocytes. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | Effects of T-2 toxin on the primary cultures of adult rat hepatocytes | - |
dc.title.alternative | 일차배양 간세포 에서의 T-2 독소의 영향에 관한 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 64049/325007 | - |
dc.description.department | 한국과학기술원 : 생물공학과, | - |
dc.identifier.uid | 000821093 | - |
dc.contributor.localauthor | Yang, K. H. | - |
dc.contributor.localauthor | 양규환 | - |
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