DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, In Young | ko |
dc.contributor.author | Lim, HoTae | ko |
dc.contributor.author | Huynh, Alex | ko |
dc.contributor.author | Schofield, James | ko |
dc.contributor.author | Cho, Hyeon Jin | ko |
dc.contributor.author | Lee, Hosuk | ko |
dc.contributor.author | Andersen, Peter | ko |
dc.contributor.author | Shin, Joo Heon | ko |
dc.contributor.author | Heo, Won Do | ko |
dc.contributor.author | Hyun, Sang-Hwan | ko |
dc.contributor.author | Kim, Yong Jun | ko |
dc.contributor.author | Oh, Yohan | ko |
dc.contributor.author | Kim, Hyesoo | ko |
dc.contributor.author | Lee, Gabsang | ko |
dc.date.accessioned | 2021-03-17T05:10:23Z | - |
dc.date.available | 2021-03-17T05:10:23Z | - |
dc.date.created | 2021-03-17 | - |
dc.date.created | 2021-03-17 | - |
dc.date.issued | 2021-02 | - |
dc.identifier.citation | BIOMATERIALS, v.269 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | http://hdl.handle.net/10203/281593 | - |
dc.description.abstract | Stem cell fate is largely determined by cellular signaling networks and is heavily dependent on the supplementation of exogenous recombinant proteins into culture media; however, uneven distribution and inconsistent stability of recombinant proteins are closely associated with the spontaneous differentiation of pluripotent stem cells (PSCs) and result in significant costs in large-scale manufacturing. Here, we report a novel PSC culture system via wirelessly controllable optical activation of the fibroblast growth factor (FGF) signaling pathway without the need for supplementation of recombinant FGF2 protein, a key molecule for maintaining pluripotency of PSCs. Using a fusion protein between the cytoplasmic region of the FGF receptor-1 and a light-oxygen-voltage domain, we achieved tunable, blue light-dependent activation of FGF signaling in human and porcine PSCs. Our data demonstrate that a highly controllable optical stimulation of the FGF signaling pathway is sufficient for long-term maintenance of PSCs, without the loss of differentiation potential into three germ layers. This culture system will be a cost-effective platform for a large-scale stem cell culture. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Novel culture system via wirelessly controllable optical stimulation of the FGF signaling pathway for human and pig pluripotency | - |
dc.type | Article | - |
dc.identifier.wosid | 000617938500002 | - |
dc.identifier.scopusid | 2-s2.0-85088800536 | - |
dc.type.rims | ART | - |
dc.citation.volume | 269 | - |
dc.citation.publicationname | BIOMATERIALS | - |
dc.identifier.doi | 10.1016/j.biomaterials.2020.120222 | - |
dc.contributor.localauthor | Heo, Won Do | - |
dc.contributor.nonIdAuthor | Choi, In Young | - |
dc.contributor.nonIdAuthor | Lim, HoTae | - |
dc.contributor.nonIdAuthor | Huynh, Alex | - |
dc.contributor.nonIdAuthor | Schofield, James | - |
dc.contributor.nonIdAuthor | Cho, Hyeon Jin | - |
dc.contributor.nonIdAuthor | Lee, Hosuk | - |
dc.contributor.nonIdAuthor | Andersen, Peter | - |
dc.contributor.nonIdAuthor | Shin, Joo Heon | - |
dc.contributor.nonIdAuthor | Hyun, Sang-Hwan | - |
dc.contributor.nonIdAuthor | Kim, Yong Jun | - |
dc.contributor.nonIdAuthor | Oh, Yohan | - |
dc.contributor.nonIdAuthor | Kim, Hyesoo | - |
dc.contributor.nonIdAuthor | Lee, Gabsang | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Optogenetics | - |
dc.subject.keywordAuthor | FGF2 | - |
dc.subject.keywordAuthor | FGF signaling | - |
dc.subject.keywordAuthor | Pluripotent stem cell | - |
dc.subject.keywordAuthor | Pluripotency | - |
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