Variable for the production of inulase using jerusalem artichoke media

Abstract

$\mbox{\underline{Kluyveromyces}}$ $\mbox{\underline{fragilis}}$ No. 351 was used to study the optimum conditions for inulase production. In order to maximize the production of inulase, the composition of the medium containing Jerusalem artichoke tuber extract were studied. The optimum concentration of Jerusalem artichoke tuber was 3.5\% (dry weight) and bactocasitone was better than yeast extract. The optimum concentration of bactocasitone was 2.0\% and the optimum temperature \& pH of the production of inulase were 30$^\circ$C and pH 5.5 respectively. The addition of $NH_4H_2PO_4$ (0.5\%) increased the production of inulase, and the effect of various metal salt was also studied. The requirement of metal salt was KCl (40 ppm), $MgSO_4\cdot7H_2O$ (20 ppm), $FeSO_4\cdot7H_2O$ (20 ppm), NaCl (20 ppm), $CoCl_2\cdot6H_2O$ (30 ppm). Inulase synthesis was growth - associated (intracellular) and the production of inulase was increased as the concentration of dissolved oxygen concentration was increasing. The addition of Tween 85 increased extracellular inulase but antifoam decreased. Maximum production of inulase was 3.3 unit/ml (Total inulase). A high quantity of industrial grade inulase was prepared by autolysis of the yeast cells, ultrafiltration of supernatant and ethanol fractionation. The recovery was 72\%.