DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Park, Tae-Gwan | - |
dc.contributor.advisor | 박태관 | - |
dc.contributor.author | Jung, Soo-Yeon | - |
dc.contributor.author | 정수연 | - |
dc.date.accessioned | 2011-12-12T08:55:16Z | - |
dc.date.available | 2011-12-12T08:55:16Z | - |
dc.date.issued | 2010 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=419048&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28114 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생명과학과, 2010.2, [ v, 56 p. ] | - |
dc.description.abstract | Small interfering RNA (siRNA) was conjugated with poly(ethylene glycol) (PEG) at four different terminal ends (sense 3’, sense 5’, antisense 3’, and antisense 5’) via a cleavable disulfide and a noncleavable thioether linkage to evaluate their gene silencing efficiencies upon complexation with $Lipofectamine^{TM}$2000. The PEGylation site at the four siRNA termini and PEG molecular weight were not a critical factor to significantly affect gene silencing activities. Cleavable siRNA-PEG conjugates showed comparable gene silencing activities to naked siRNA, and exhibited sequence-specific degradation of a target mRNA. Interestingly, noncleavable siRNA-PEG conjugates were processed by Dicer, enabling to exert RNAi effect with demonstrating non-specific mRNA degradation. However, only cleavable siRNA-PEG conjugates significantly reduced the extent of $INF-\alpha$ release as compared to noncleavable siRNA-PEG conjugates, suggesting that they can be potentially used for therapeutic siRNA applications. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | PEG | - |
dc.subject | siRNA | - |
dc.subject | Dicer | - |
dc.subject | Dicer | - |
dc.subject | PEG | - |
dc.subject | 소간섭RNA | - |
dc.title | Gene silencing efficiency of siRNA-PEG conjugates | - |
dc.title.alternative | PEGylation site와 PEG 분자량이 siRNA-PEG conjugate의 유전자 발현 억제에 미치는 영향에 관한 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 419048/325007 | - |
dc.description.department | 한국과학기술원 : 생명과학과, | - |
dc.identifier.uid | 020088057 | - |
dc.contributor.localauthor | Park, Tae-Gwan | - |
dc.contributor.localauthor | 박태관 | - |
dc.title.subtitle | effect of PEGylation site and PEG molecular weight | - |
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