DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kim, Sun-Chang | - |
dc.contributor.advisor | 김선창 | - |
dc.contributor.author | Yoo, Hee-Kyeom | - |
dc.contributor.author | 유희겸 | - |
dc.date.accessioned | 2011-12-12T08:54:54Z | - |
dc.date.available | 2011-12-12T08:54:54Z | - |
dc.date.issued | 2007 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=264267&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28089 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생명과학과, 2007.2, [ iv, 35 p. ] | - |
dc.description.abstract | Based on whole-genome sequences, strain reconstruction studies for improved industrial applications have been initiated. For the construction of a custom-designed microorganism, an efficient genetic engineering tool which can transfer large genomic segments carrying useful genomic information is needed. We developed a novel and efficient method called genome swapping using Cre/lox site-specific recombination and a γ-ori replication system that can transfer large genomic segments between various microorganisms. In this study, we attempted to construct an IS-free Escherichia coli B strain, which is a popular industrial host strain in the production of useful biomaterials. However, mobile DNA elements such as insertion sequence elements (IS) and transposases caused a genetic unstableness. To eliminate this problem, we targeted IS elements for removal in 5 target regions and deleted IS elements within these target regions. The genome swapping method may be used to transfer IS-deleted target regions of the E. coli chromosome in one cell for the rapid construction of an IS-free E. coli B strain. Also, it is possible to construct a minimized genome and a hybrid microorganism for use in bioindustry and biotechnology. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Cre/lox site specific recombination | - |
dc.subject | large fragment | - |
dc.subject | genome swapping | - |
dc.subject | hybrid | - |
dc.subject | 대장균 B 균주 | - |
dc.subject | 하이브리드 | - |
dc.subject | Cre/lox 재조합 | - |
dc.subject | 유전체 치환기술 | - |
dc.subject | E.coli B Strain | - |
dc.title | Development of a genome swapping method and its application for hybrid microorganisms | - |
dc.title.alternative | 미생물 유전체 치환기술 개발 및 이를 이용한 하이브리드 균주 구축 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 264267/325007 | - |
dc.description.department | 한국과학기술원 : 생명과학과, | - |
dc.identifier.uid | 020053378 | - |
dc.contributor.localauthor | Kim, Sun-Chang | - |
dc.contributor.localauthor | 김선창 | - |
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