Development of a genome swapping method and its application for hybrid microorganisms미생물 유전체 치환기술 개발 및 이를 이용한 하이브리드 균주 구축

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dc.contributor.advisorKim, Sun-Chang-
dc.contributor.advisor김선창-
dc.contributor.authorYoo, Hee-Kyeom-
dc.contributor.author유희겸-
dc.date.accessioned2011-12-12T08:54:54Z-
dc.date.available2011-12-12T08:54:54Z-
dc.date.issued2007-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=264267&flag=dissertation-
dc.identifier.urihttp://hdl.handle.net/10203/28089-
dc.description학위논문(석사) - 한국과학기술원 : 생명과학과, 2007.2, [ iv, 35 p. ]-
dc.description.abstractBased on whole-genome sequences, strain reconstruction studies for improved industrial applications have been initiated. For the construction of a custom-designed microorganism, an efficient genetic engineering tool which can transfer large genomic segments carrying useful genomic information is needed. We developed a novel and efficient method called genome swapping using Cre/lox site-specific recombination and a γ-ori replication system that can transfer large genomic segments between various microorganisms. In this study, we attempted to construct an IS-free Escherichia coli B strain, which is a popular industrial host strain in the production of useful biomaterials. However, mobile DNA elements such as insertion sequence elements (IS) and transposases caused a genetic unstableness. To eliminate this problem, we targeted IS elements for removal in 5 target regions and deleted IS elements within these target regions. The genome swapping method may be used to transfer IS-deleted target regions of the E. coli chromosome in one cell for the rapid construction of an IS-free E. coli B strain. Also, it is possible to construct a minimized genome and a hybrid microorganism for use in bioindustry and biotechnology.eng
dc.languageeng-
dc.publisher한국과학기술원-
dc.subjectCre/lox site specific recombination-
dc.subjectlarge fragment-
dc.subjectgenome swapping-
dc.subjecthybrid-
dc.subject대장균 B 균주-
dc.subject하이브리드-
dc.subjectCre/lox 재조합-
dc.subject유전체 치환기술-
dc.subjectE.coli B Strain-
dc.titleDevelopment of a genome swapping method and its application for hybrid microorganisms-
dc.title.alternative미생물 유전체 치환기술 개발 및 이를 이용한 하이브리드 균주 구축-
dc.typeThesis(Master)-
dc.identifier.CNRN264267/325007 -
dc.description.department한국과학기술원 : 생명과학과, -
dc.identifier.uid020053378-
dc.contributor.localauthorKim, Sun-Chang-
dc.contributor.localauthor김선창-
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