DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kang, Chang-Won | - |
dc.contributor.advisor | 강창원 | - |
dc.contributor.author | Park, Sung-Ho | - |
dc.contributor.author | 박성호 | - |
dc.date.accessioned | 2011-12-12T08:54:13Z | - |
dc.date.available | 2011-12-12T08:54:13Z | - |
dc.date.issued | 2005 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=243517&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28044 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생명과학과, 2005.2, [ vi, 44 p. ] | - |
dc.description.abstract | We report a multiplex analysis approach using mass-tagged extension primers in single base extension for genotyping of single nucleotide polymorphism (SNP). In this method, oligonucleotide primers that are specific to polymorphic sites in the DNA template are simultaneously extended with each biotin-ddNTP by DNA polymerase to generate 3’-biotinylated DNA products in each four tube. These products in each tube are captured by streptoavidin-coated magnetic beads while unextended primers and other components are washed away. These captured extended primers which have one deoxyuridine sequence between 5’-mass-tag region and 3’-template recognition region are cut by uracil-DNA glycosylase to generate liberal oligonucleotides that have distinct mass according to specific SNP sites. The liberal oligonucleotides which code specific SNP sites in each tube are identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). We successfully analyzed 50 SNP loci at the same time. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | MALDI-TOF SNP multiplex genotyping single nucleotide polymorphismn | - |
dc.subject | step-in mode of AFM | - |
dc.subject | 주파수 분석 | - |
dc.subject | 질량분석기 다병렬 분석 단일염기다형성 분석방법 | - |
dc.subject | frequency analysis | - |
dc.title | Multiplex genotyping of single nucleotide polymorphisms using MALDI-TOF mass spectrometry | - |
dc.title.alternative | MALDI-TOF 질량분석기를 이용한 단일염기다형성 다병렬 분석 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 243517/325007 | - |
dc.description.department | 한국과학기술원 : 생명과학과, | - |
dc.identifier.uid | 020033246 | - |
dc.contributor.localauthor | Kang, Chang-Won | - |
dc.contributor.localauthor | 강창원 | - |
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