DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Chung, Won-Il | - |
dc.contributor.advisor | 정원일 | - |
dc.contributor.author | Kim, Joon | - |
dc.contributor.author | 김준 | - |
dc.date.accessioned | 2011-12-12T08:53:09Z | - |
dc.date.available | 2011-12-12T08:53:09Z | - |
dc.date.issued | 2002 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=177022&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/27974 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물과학과, 2002.8, [ iii, 30 p. ] | - |
dc.description.abstract | Elimination of selectable marker genes is essential technique for the relaxation of consumer concerns on genetically modified organisms (GMOs) and gene stacking. In this study, a self-removable selectable marker cassette consists of glucocorticoid-regulated Cre/loxP recombination system, nptii and a negative selection marker cytosine deaminase (codA) gene was constructed between the strong plant promoter CaMV 35S promoter and a β-glucuronidase (GUS) gene. By the induction of cre expression with glucocorticoid hormone dexamethasone, the cassette was eliminated and GUS reporter gene was activated. The eliminated region was confirmed by PCR. Cells still carrying selectable markers were killed by codA that converts non-toxic 5-fluorocytosine to toxic 5-fluorouracil. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | plant transformation | - |
dc.subject | codA | - |
dc.subject | cytosine deaminase | - |
dc.subject | cre/loxP | - |
dc.subject | selectable marker elimination | - |
dc.subject | 유전자 제거 | - |
dc.subject | 선발표지 유전자 | - |
dc.subject | 시토신 디아미나제 | - |
dc.subject | 벡터 제작 | - |
dc.subject | 식물형질전환 | - |
dc.title | Elimination of selectable marker genes with chemical-inducible cre/loxP system and cytosine deaminase in plant | - |
dc.title.alternative | 식물에서 Cre/loxP 시스템과 cytosine deaminase를 이용한 선발표지 유전자의 제거 기법 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 177022/325007 | - |
dc.description.department | 한국과학기술원 : 생물과학과, | - |
dc.identifier.uid | 020003807 | - |
dc.contributor.localauthor | Chung, Won-Il | - |
dc.contributor.localauthor | 정원일 | - |
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