DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Lee, Gyun-Min | - |
dc.contributor.advisor | 이균민 | - |
dc.contributor.author | Lee, Suk-Kyoo | - |
dc.contributor.author | 이석규 | - |
dc.date.accessioned | 2011-12-12T08:52:59Z | - |
dc.date.available | 2011-12-12T08:52:59Z | - |
dc.date.issued | 2002 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=173565&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/27963 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물과학과, 2002.2, [ iv, 42 p ] | - |
dc.description.abstract | Chinese Hamster Ovary (CHO) dhfr(-) cell line overexpressing anti-apoptotic protein Bcl-2, CHO dhfr(-) Bcl2. was developed for the establishment of recombinant CHO cells producing humanized antibody. CHO dhfr(-) Bcl2 stably overexpressed Bcl-2 protein without selection pressure and exhibited delay of apoptotic cell death in serum-free suspension culture. Established recombinant cell lines producing humanized antibody using CHO dhfr(-) Bcl2, CHO Bcl2-19-008 and CHO Bcl2-24-008, exhibited extended culture longevity in batch culture of serum-free suspension than control cell lines, CHO neo-04-008 and CHO neo-24-008. In stressful condition induced by 5mM NaBu addition, final MAb titers of CHO Bcl2-19-008 and CHO Bcl2-24-008 increased to $50㎍/mL^{-1}$, $11 ㎍/mL^{-1}$, which were five fold, three fold of final MAb titers in serum-free suspension culture, respectively. Compared with cell lines developed from CHO dhfr(-) Bcl2, CHO neo-04-008 and CHO neo-24-008 showed no significant increase of final MAb titer because rapid decline fo cell viablility after NaBa addition | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | CHO cell line | - |
dc.subject | apoptosis | - |
dc.subject | host cell line | - |
dc.subject | 숙주 세포주 | - |
dc.subject | 유전자 증폭 | - |
dc.subject | 세포예정사 | - |
dc.subject | gene amplification | - |
dc.title | Anti-apoptotic CHO dhfr(-) host cell line | - |
dc.title.alternative | 항 세포예정사 CHO dhfr(-) 숙주 세포주 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 173565/325007 | - |
dc.description.department | 한국과학기술원 : 생물과학과, | - |
dc.identifier.uid | 020003386 | - |
dc.contributor.localauthor | Lee, Gyun-Min | - |
dc.contributor.localauthor | 이균민 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.