DC Field | Value | Language |
---|---|---|
dc.contributor.author | Han, Taehee | ko |
dc.contributor.author | Kim, Gi Bae | ko |
dc.contributor.author | Lee, Sang Yup | ko |
dc.date.accessioned | 2020-12-10T08:30:10Z | - |
dc.date.available | 2020-12-10T08:30:10Z | - |
dc.date.created | 2020-12-01 | - |
dc.date.created | 2020-12-01 | - |
dc.date.issued | 2020-12 | - |
dc.identifier.citation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.117, no.48, pp.30328 - 30334 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/10203/278140 | - |
dc.description.abstract | Contributed by Sang Yup Lee, October 6, 2020 (sent for review August 18, 2020; There is increasing industrial demand for five-carbon platform chemicals, particularly glutaric acid, a widely used building block chemical for the synthesis of polyesters and polyamides. Here we report the development of an efficient glutaric acid microbial producer by systems metabolic engineering of an L-lysine-overproducing Corynebacterium glutamicum BE strain. Based on our previous study, an optimal synthetic metabolic pathway comprising Pseudomonas putida L-lysine monooxygenase (davB) and 5-aminovaleramide amidohydrolase (davA) genes and C. glutamicum 4-aminobutyrate aminotransferase (gabT) and succinate-semialdehyde dehydrogenase (gabD) genes, was introduced into the C. glutamicum BE strain. Through system-wide analyses including genome-scale metabolic simulation, comparative transcriptome analysis, and flux response analysis, 11 target genes to be manipulated were identified and expressed at desired levels to increase the supply of direct precursor L-lysine and reduce precursor loss. A glutaric acid exporter encoded by ynfM was discovered and overexpressed to further enhance glutaric acid production. Fermentation conditions, including oxygen transfer rate, batch-phase glucose level, and nutrient feeding strategy, were optimized for the efficient production of glutaric acid. Fed-batch culture of the final engineered strain produced 105.3 g/L of glutaric acid in 69 h without any byproduct. The strategies of metabolic engineering and fermentation optimization described here will be useful for developing engineered microorganisms for the high-level bio-based production of other chemicals of interest to industry. | - |
dc.language | English | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.title | Glutaric acid production by systems metabolic engineering of an l-lysine–overproducing Corynebacterium glutamicum | - |
dc.type | Article | - |
dc.identifier.wosid | 000596566400007 | - |
dc.identifier.scopusid | 2-s2.0-85097210416 | - |
dc.type.rims | ART | - |
dc.citation.volume | 117 | - |
dc.citation.issue | 48 | - |
dc.citation.beginningpage | 30328 | - |
dc.citation.endingpage | 30334 | - |
dc.citation.publicationname | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.identifier.doi | 10.1073/pnas.2017483117 | - |
dc.contributor.localauthor | Lee, Sang Yup | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | metabolic engineering | - |
dc.subject.keywordAuthor | Corynebacterium glutamicum | - |
dc.subject.keywordAuthor | glutaric acid | - |
dc.subject.keywordAuthor | multiomics | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | 5-AMINOVALERATE | - |
dc.subject.keywordPlus | CATABOLISM | - |
dc.subject.keywordPlus | PATHWAYS | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.