Balanced Detection Method Using Optical Affinity Sensors for Quick Measurement of Biomolecule Concentrations

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To determine the concentration of biomolecules using a label-free optical biosensor, it is necessary to measure the serial signal from the reaction starting point, which is inconvenient for practical applications. Here, we propose an alternative detection method for determining the concentration of a biomolecule. The method, which is derived from the fraction bound equation of the Langmuir adsorption model, determines the concentration relative to a reference sample with required accuracy, with a single measurement at any point in time. We also experimentally demonstrated the method and its accuracy by detecting streptavidin-biotin complexes using on-chip optical sensors based on active disk resonators integrated with microfluidic circuits. By performing the proposed method in a simultaneous parallel measurement scheme, signal fluctuations evenly induced in the detectors by external perturbations could be automatically suppressed, similar to the balanced detection method. We expect our approach to be applicable to practical applications where fast and accurate detection responses are needed.
Publisher
AMER CHEMICAL SOC
Issue Date
2020-05
Language
English
Article Type
Article
Citation

ANALYTICAL CHEMISTRY, v.92, no.9, pp.6189 - 6193

ISSN
0003-2700
DOI
10.1021/acs.analchem.0c00637
URI
http://hdl.handle.net/10203/274304
Appears in Collection
NT-Journal Papers(저널논문)
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