DC Field | Value | Language |
---|---|---|
dc.contributor.author | Min, Kyeongbae | ko |
dc.contributor.author | Cho, In | ko |
dc.contributor.author | Choi, Myunghwan | ko |
dc.contributor.author | Chang, Jae-Byum | ko |
dc.date.accessioned | 2020-05-06T06:20:26Z | - |
dc.date.available | 2020-05-06T06:20:26Z | - |
dc.date.created | 2019-12-25 | - |
dc.date.issued | 2020-03 | - |
dc.identifier.citation | METHODS, v.174, pp.3 - 10 | - |
dc.identifier.issn | 1046-2023 | - |
dc.identifier.uri | http://hdl.handle.net/10203/274100 | - |
dc.description.abstract | Super-resolution microscopy techniques have been widely adopted in biological sciences. Recently, a new super-resolution microscopy technique, called expansion microscopy (ExM) has been developed. In this technique, biomolecules inside specimens are first labeled with fluorophores, followed by in-situ hydrogel synthesis and physical expansion of the specimens. Image quality, including brightness and signal-to-noise ratio, depends on the extent to which fluorophores have bleached during the in-situ hydrogel synthesis process. In this work, we compared the fluorescence signal brightness of more than 20 fluorophores, after expansion, to identify the best fluorophore set for 4-color expansion microscopy imaging. In addition, we achieved 5-color multiplexed expansion microscopy by photo-bleaching one of the four fluorophores and re-staining thereafter. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Multiplexed expansion microscopy of the brain through fluorophore screening | - |
dc.type | Article | - |
dc.identifier.wosid | 000525860400002 | - |
dc.identifier.scopusid | 2-s2.0-85071376937 | - |
dc.type.rims | ART | - |
dc.citation.volume | 174 | - |
dc.citation.beginningpage | 3 | - |
dc.citation.endingpage | 10 | - |
dc.citation.publicationname | METHODS | - |
dc.identifier.doi | 10.1016/j.ymeth.2019.07.017 | - |
dc.contributor.localauthor | Chang, Jae-Byum | - |
dc.contributor.nonIdAuthor | Min, Kyeongbae | - |
dc.contributor.nonIdAuthor | Cho, In | - |
dc.contributor.nonIdAuthor | Choi, Myunghwan | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | PROTEIN | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.