DC Field | Value | Language |
---|---|---|
dc.contributor.author | Martino, Nicola | ko |
dc.contributor.author | Kwok, Sheldon J. J. | ko |
dc.contributor.author | Liapis, Andreas C. | ko |
dc.contributor.author | Forward, Sarah | ko |
dc.contributor.author | Jang, Hoon | ko |
dc.contributor.author | Kim, Hwi-Min | ko |
dc.contributor.author | Wu, Sarah J. | ko |
dc.contributor.author | Wu, Jiamin | ko |
dc.contributor.author | Dannenberg, Paul H. | ko |
dc.contributor.author | Lee, Yong-Hee | ko |
dc.contributor.author | Jang, Sun-Joo | ko |
dc.contributor.author | Yun, Seok-Hyun | ko |
dc.date.accessioned | 2019-10-15T01:20:12Z | - |
dc.date.available | 2019-10-15T01:20:12Z | - |
dc.date.created | 2019-10-14 | - |
dc.date.created | 2019-10-14 | - |
dc.date.issued | 2019-10 | - |
dc.identifier.citation | NATURE PHOTONICS, v.13, no.10, pp.720 - + | - |
dc.identifier.issn | 1749-4885 | - |
dc.identifier.uri | http://hdl.handle.net/10203/267976 | - |
dc.description.abstract | Large-scale single-cell analyses have become increasingly important given the role of cellular heterogeneity in complex biological systems. However, no techniques at present enable optical imaging of uniquely tagged individual cells. Fluorescence-based approaches can distinguish only a small number of distinct cells or cell groups at a time because of spectral crosstalk between conventional fluorophores. Here we investigate large-scale cell tracking using intracellular laser particles as imaging probes that emit coherent laser light with a characteristic wavelength. Made of silica-coated semiconductor microcavities, these laser particles have single-mode emission over a broad range from 1,170 nm to 1,580 nm with sub-nanometre linewidths, enabling massive spectral multiplexing. We explore the stability and biocompatibility of these probes in vitro and their utility for wavelength-multiplexed cell tagging and imaging. We demonstrate real-time tracking of thousands of individual cells in a three-dimensional tumour model over several days, showing different behavioural phenotypes. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Wavelength-encoded laser particles for massively multiplexed cell tagging | - |
dc.type | Article | - |
dc.identifier.wosid | 000487333400013 | - |
dc.identifier.scopusid | 2-s2.0-85070323788 | - |
dc.type.rims | ART | - |
dc.citation.volume | 13 | - |
dc.citation.issue | 10 | - |
dc.citation.beginningpage | 720 | - |
dc.citation.endingpage | + | - |
dc.citation.publicationname | NATURE PHOTONICS | - |
dc.identifier.doi | 10.1038/s41566-019-0489-0 | - |
dc.contributor.localauthor | Lee, Yong-Hee | - |
dc.contributor.nonIdAuthor | Martino, Nicola | - |
dc.contributor.nonIdAuthor | Kwok, Sheldon J. J. | - |
dc.contributor.nonIdAuthor | Liapis, Andreas C. | - |
dc.contributor.nonIdAuthor | Forward, Sarah | - |
dc.contributor.nonIdAuthor | Jang, Hoon | - |
dc.contributor.nonIdAuthor | Kim, Hwi-Min | - |
dc.contributor.nonIdAuthor | Wu, Sarah J. | - |
dc.contributor.nonIdAuthor | Wu, Jiamin | - |
dc.contributor.nonIdAuthor | Dannenberg, Paul H. | - |
dc.contributor.nonIdAuthor | Jang, Sun-Joo | - |
dc.contributor.nonIdAuthor | Yun, Seok-Hyun | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | COATED GOLD NANOPARTICLES | - |
dc.subject.keywordPlus | CLONAL DYNAMICS | - |
dc.subject.keywordPlus | REVEALS | - |
dc.subject.keywordPlus | MONODISPERSE | - |
dc.subject.keywordPlus | EXPRESSION | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.