DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Dong Young | ko |
dc.contributor.author | Park, Jeong Ae | ko |
dc.contributor.author | Kim, Yeomyung | ko |
dc.contributor.author | Noh, Minyoung | ko |
dc.contributor.author | Park, Songyi | ko |
dc.contributor.author | Lie, Eunkyung | ko |
dc.contributor.author | Kim, Eunjoon | ko |
dc.contributor.author | Kim, Young-Myeong | ko |
dc.contributor.author | Kwon, Young-Guen | ko |
dc.date.accessioned | 2019-09-10T02:20:03Z | - |
dc.date.available | 2019-09-10T02:20:03Z | - |
dc.date.created | 2019-09-09 | - |
dc.date.created | 2019-09-09 | - |
dc.date.issued | 2019-09 | - |
dc.identifier.citation | FASEB JOURNAL, v.33, no.9, pp.9842 - 9857 | - |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | http://hdl.handle.net/10203/267424 | - |
dc.description.abstract | Angiogenesis depends on VEGF-mediated signaling. However, the regulatory mechanisms and functions of individual VEGF receptor 2 (VEGFR2) phosphorylation sites remain unclear. Here, we report that synaptic adhesion-like molecule 4 (SALM4) regulates a specific VEGFR2 phosphorylation site. SALM4 silencing in HUVECs and Salm4 knockout (KO) in lung endothelial cells (ECs) of Salm4(-/-) mice suppressed phosphorylation of VEGFR2 tyrosine (Y) 1175 (Y1173 in mice) and downstream signaling upon VEGF-A stimulation. However, VEGFR2 phosphorylation at Y951 (Y949 in mice) and Y1214 (Y1212 in mice) remained unchanged. Knockdown and KO of SALM4 inhibited VEGF-A-induced angiogenic functions of ECs. SALM4 depletion reduced endothelial leakage, sprouting, and migratory activities. Furthermore, in an ischemia and reperfusion (I/R) model, brain injury was attenuated in Salm4(-/-) mice compared with wild-type (WT) mice. In brain lysates after I/R, VEGFR2 phosphorylation at Y949, Y1173, and Y1212 were induced in WT brains, but only Y1173 phosphorylation of VEGFR2 was reduced in Salm4(-/-) brains. Taken together, our results demonstrate that SALM4 specifically regulates VEGFR2 phosphorylation at Y1175 (Y1173 in mice), thereby fine-tuning VEGF signaling in ECs.-Kim, D. Y., Park, J. A., Kim, Y., Noh, M., Park, S., Lie, E., Kim, E., Kim, Y.-M., Kwon, Y.-G. SALM4 regulates angiogenic functions in endothelial cells through VEGFR2 phosphorylation at Tyr1175. | - |
dc.language | English | - |
dc.publisher | FEDERATION AMER SOC EXP BIOL | - |
dc.title | SALM4 regulates angiogenic functions in endothelial cells through VEGFR2 phosphorylation at Tyr1175 | - |
dc.type | Article | - |
dc.identifier.wosid | 000482214200013 | - |
dc.identifier.scopusid | 2-s2.0-85071786466 | - |
dc.type.rims | ART | - |
dc.citation.volume | 33 | - |
dc.citation.issue | 9 | - |
dc.citation.beginningpage | 9842 | - |
dc.citation.endingpage | 9857 | - |
dc.citation.publicationname | FASEB JOURNAL | - |
dc.identifier.doi | 10.1096/fj.201802516RR | - |
dc.contributor.localauthor | Kim, Eunjoon | - |
dc.contributor.nonIdAuthor | Kim, Dong Young | - |
dc.contributor.nonIdAuthor | Park, Jeong Ae | - |
dc.contributor.nonIdAuthor | Kim, Yeomyung | - |
dc.contributor.nonIdAuthor | Noh, Minyoung | - |
dc.contributor.nonIdAuthor | Park, Songyi | - |
dc.contributor.nonIdAuthor | Kim, Young-Myeong | - |
dc.contributor.nonIdAuthor | Kwon, Young-Guen | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | VEGF signaling | - |
dc.subject.keywordAuthor | endothelial leakage | - |
dc.subject.keywordAuthor | vascular permeability | - |
dc.subject.keywordAuthor | ischemia reperfusion | - |
dc.subject.keywordPlus | VASCULAR-PERMEABILITY | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | ISCHEMIC-STROKE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | FAMILY | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | SURVIVAL | - |
dc.subject.keywordPlus | BIOLOGY | - |
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