Generation and expansion of functional cardioblasts from pluripotent stem cells for cardiac regeneration심장 재생을 위한 줄기세포로부터 기능성 심장근아세포의 생성 및 증식에 관한 연구

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Isolating actively proliferating cardioblasts is the first crucial step for cardiac regeneration through cell implantation. However, the origin and identity of putative cardioblasts are still unclear. Here, I uncover a novel class of cardiac lineage cells, $PDGFRα^+ Flk1^-$ cardioblasts (PCBs), from mouse and human pluripotent stem cells induced using CsAYTE, a combination of the small molecules Cyclosporin A, the rho-associated coiled-coil kinase inhibitor Y27632, the antioxidant Trolox, and the ALK5 inhibitor EW7197. This novel population of actively proliferating cells is cardiac lineage-committed but in a morphologically and functionally immature state compared to mature cardiomyocytes. Most important, most of CsAYTE-induced PCBs spontaneously differentiated into functional $αMHC^+$ cardiomyocytes ($M^+CMs$) and could be a potential cellular resource for cardiac regeneration. Indeed, not only PCB-derived $M^+CMs$ but also undifferentiated PCBs effectively restored cardiac systolic function in a murine model of acute myocardial infarction through proper integration into the host myocardium.
Advisors
Koh, Gou Youngresearcher고규영researcher
Description
한국과학기술원 :의과학학제전공,
Publisher
한국과학기술원
Issue Date
2018
Identifier
325007
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 의과학학제전공, 2018.2,[iv, 75 p. :]

Keywords

cardioblast▼apluripotent stem cells▼acardiac differentiation▼acardiac stem cells▼acardiac regeneration; 심장근아세포▼a다능성줄기세포▼a심장 분화▼a심장 줄기세포▼a심장재생

URI
http://hdl.handle.net/10203/265109
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=734377&flag=dissertation
Appears in Collection
MSE-Theses_Ph.D.(박사논문)
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