Role of cerebellar Shank2 in the regulation of synaptic and behavioral functions

Abstract

Shank2 is a multidomain scaffolding protein implicated in the structural and functional coordination of multiprotein complexes at excitatory postsynaptic sites as well as in psychiatric disorders, including autism spectrum disorders. While Shank2 is strongly expressed in the cerebellum, whether Shank2 regulates cerebellar excitatory synapses, or contributes to the behavioral abnormalities observed in $Shank2^{-/-}$ mice, remains unexplored. Here we show that $Shank2^{-/-}$ mice show reduced excitatory synapse density in cerebellar Purkinje cells in association with reduced levels of excitatory postsynaptic proteins including GluD2 and PSD-93 and impaired motor coordination in the Erasmus test. Shank2 deletion restricted to Purkinje cells ($Pcp2-Cre

Shank2^{fl/fl}$ mice) leads to similar reductions in excitatory synapse density, synaptic protein levels, and motor coordination. $Pcp2-Cre

Shank2^{fl/fl}$ mice do not recapitulate autistic-like behaviors observed in $Shank2^{-/-}$ mice, such as social-interaction deficits, altered ultrasonic vocalizations, repetitive behaviors, and hyperactivity. However, $Pcp2-Cre

Shank2^{fl/fl}$ mice display enhanced repetitive behavior in the hole-board test and anxiety-like behavior in the light-dark test, which are not observed in $Shank2^{-/-}$ mice. These results implicate Shank2 in the regulation of cerebellar excitatory synapse density, motor coordination, and specific repetitive and anxiety-like behaviors.