DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kim, Sun Chang | - |
dc.contributor.advisor | 김선창 | - |
dc.contributor.author | Cui, Chang-hao | - |
dc.date.accessioned | 2019-08-22T02:43:22Z | - |
dc.date.available | 2019-08-22T02:43:22Z | - |
dc.date.issued | 2015 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=868084&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/264766 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생명과학과, 2015.2,[ix, 126 p. :] | - |
dc.description.abstract | Ginsenosides, which are isolated from ginseng, have been regarded as the principal constituents re-sponsible for the pharmacological activities. They can be categorized as protopanaxadiol (PPD) type, pro-topanaxatriol (PPT) type, and oleanane saponins, based on the structure of the aglycon, with a dammarane skeleton. The six major ginsenosides ($Rb_1$, $Rb_2$, $R_c$, $R_d$, $R_e$, and $Rg_1$) constitute more than 80% of the total ginsenosides in ginseng root, and minor ginsenosides that are deglycosylated from the major ginsenosides exist in smaller amounts in the ginseng. Emerging evidence has demonstrated that the minor ginsenosides have more profound pharmaceutical activities than the glycosylated major ginsenosides. The production of minor ginsenosides from the major ginsenosides has been accomplished through physiochemical and micro-biological methods. However, various enzymatic methods have been reported thus far for the preparation of minor ginsenosides, as a result of their higher conversion efficiency, fewer by-products, superior environmen-tal protection, and better stereo-specificity. For the mass production of minor ginsenosides from major gin-senosides, tens of ginsenosides-transforming glycoside hydrolases from soil bacteria and putative genes were cloned and characterized for ginsenoside-transforming ability. Among them, 3 $\beta$-glucosidases from Acti-nosynnema mirum (BglAm), Paenibacillus mucilaginosus (BglPm) and Microbacterium sp. Gsoil 167 (Bgl167b) | - |
dc.description.abstract | an α-L-rhamnosidase from Bifidobacterium dentium BD1 (BD890) were characterized. Using recombinant glycoside hydrolases, 100 gram-scale or gram-scale production of ginsenoside $F_2$, $Rh_1$, $Rg_2$, gypenoside LXXV and $Rg_1$ have been achieved from relative abundant ginsenosides mixtures or major gin-senosides. These results suggest that these enzymatic methods could be usefully exploited in the preparation of pharmacologically active minor ginsenosides in the cosmetics, functional food, and pharmaceutical indus-tries. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | ginsenosides▼aginseng▼adeglycosylation▼anatural product▼aglycoside hydrolases | - |
dc.subject | 진세노사이드▼a인삼▼a데글리코실화▼a천연물▼a그라이코시다제 | - |
dc.title | Mass production of minor ginsenosides for the study of their pharmacological effects | - |
dc.title.alternative | 약효연구를 위한 마이너 진세노사이드의 대량생산 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :생명과학과, | - |
dc.contributor.alternativeauthor | 최창호 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.